Majhi Sullip Kumar, Maurya Pradip Kumar, Kumar Santosh, Mohindra Vindhya, Lal Kuldeep Kumar
ICAR-National Bureau of Fish Genetic Resources, Lucknow, India.
Reprod Domest Anim. 2019 Dec;54(12):1560-1566. doi: 10.1111/rda.13564. Epub 2019 Sep 26.
Germ cell (GC) transplantation (GCT) is a proven powerful reproductive technique to enhance the production efficiency of domesticated animals and aid to the recovery of endangered germ lines. In mammals, several methods have been adopted for the eradication of GCs such as treatment with cytotoxic drugs, irradiation, cold ischaemia and hyperthermic treatment. Some of these methods have also been tried in fishes, and conditions for sterilization of gonads have been established. Here, we report the production of GC-depleted male striped catfish Pangasianodon hypophthalmus in 12 weeks by the combination of heat and chemical treatments. The cytotoxic drug busulphan (40 mg/kg) was intraperitoneally injected into the animals at 2-week intervals (six doses in total) and maintained in water at 38°C between weeks 1 and 12. The effectiveness of the treatment was assessed using gonadal index and histology. At the end of 12 weeks, very severe gonadal degeneration was observed in fish treated with the heat-chemical combination, and 100% of sampled fish (n = 5) were found devoid of endogenous GCs. On contrary, high temperature alone caused minor gonadal degeneration. Results obtained in this study suggest that endogenous GCs of large-bodied fish such as P. hypophthalmus can also be sterilized by heat and chemical treatments within a considerably short period.
生殖细胞（GC）移植（GCT）是一种经证实的强大生殖技术，可提高家畜的生产效率并有助于濒危生殖系的恢复。在哺乳动物中，已采用多种方法来消除生殖细胞，如用细胞毒性药物处理、辐射、冷缺血和热疗。其中一些方法也已在鱼类中尝试，并已确定性腺绝育的条件。在此，我们报告通过热疗和化学处理相结合的方法，在12周内成功培育出无生殖细胞的雄性条纹鲶鱼（Pangasianodon hypophthalmus）。将细胞毒性药物白消安（40毫克/千克）以两周的间隔腹腔注射到动物体内（共六剂），并在第1周和第12周期间将其饲养在38°C的水中。使用性腺指数和组织学评估处理的有效性。在12周结束时，观察到经热化学联合处理的鱼出现非常严重的性腺退化，并且发现100%的采样鱼（n = 5）没有内源性生殖细胞。相反，单独高温仅引起轻微的性腺退化。本研究获得的结果表明，像条纹鲶鱼这样的大型鱼类的内源性生殖细胞也可以在相当短的时间内通过热疗和化学处理进行绝育。
