循环促血管生成 microRNA 表达作为经皮冠状动脉介入治疗的冠心病患者快速血管造影狭窄进展和再狭窄风险的生物标志物的潜力。
Potential of circulating pro-angiogenic microRNA expressions as biomarkers for rapid angiographic stenotic progression and restenosis risks in coronary artery disease patients underwent percutaneous coronary intervention.
机构信息
Department of Cardiology, Tongji Medical College, The Central Hospital of Wuhan, Huazhong University of Science & Technology, Wuhan, China.
Emergency Department, Tongji Medical College, The Central Hospital of Wuhan, Huazhong University of Science & Technology, Wuhan, China.
出版信息
J Clin Lab Anal. 2020 Jan;34(1):e23013. doi: 10.1002/jcla.23013. Epub 2019 Sep 8.
BACKGROUND
This study aimed to investigate the correlation of pro-angiogenic microRNA (miRNA) expressions with rapid angiographic stenotic progression (RASP) and restenosis risks in coronary artery disease (CAD) patients underwent percutaneous coronary intervention (PCI) with drug-eluting stents (DES).
METHODS
A total of 286 CAD patients underwent PCI with DES were consecutively recruited in this study. Plasma samples were collected before PCI operation, and 14 pro-angiogenic miRNAs were measured by real-time quantitative reverse transcription-polymerase chain reaction. Rapid angiographic stenotic progression at nontarget lesions and restenosis at stented lesions were evaluated by quantitative coronary angiography at 12 months after PCI operation.
RESULTS
The occurrence rates of RASP and restenosis were 39.5% and 22.4%, respectively. Let-7f, miR-19a, miR-19b-1, miR-92a, miR-126, miR-210, and miR-296 were decreased in RASP patients than non-RASP patients, among which let-7f, miR-19a, miR-126, miR-210, and miR-296 independently correlated with lower RASP occurrence by multivariate analysis, followed by receiver-operating characteristic (ROC) curve exhibited that these five miRNAs showed great value in predicting RASP risk with area under curve (AUC) 0.879 (95% CI: 0.841-0.917). Besides, let-7f, miR-19a, miR-92a, miR-126, miR-130a, and miR-210 were reduced in restenosis patients than non-restenosis patients, among them miR-19a, miR-126, miR-210, and miR-378 independently correlated with lower restenosis occurrence by multivariate analysis, followed by ROC curve disclosed that these four miRNAs had good value in predicting restenosis risk with AUC 0.776 (95% CI: 0.722-0.831).
CONCLUSIONS
Circulating let-7f, miR-19a, miR-126, miR-210, and miR-296 independently correlate with reduced RASP risk, while miR-19a, miR-126, miR-210, and miR-378 independently correlate with decreased restenosis risk in CAD patients underwent PCI with DES.
背景
本研究旨在探讨促血管生成微小 RNA（miRNA）表达与经皮冠状动脉介入治疗（PCI）后药物洗脱支架（DES）置入的冠心病（CAD）患者快速血管造影狭窄进展（RASP）和再狭窄风险的相关性。
方法
本研究连续纳入 286 例接受 PCI 联合 DES 治疗的 CAD 患者。在 PCI 术前采集血浆样本，采用实时定量逆转录聚合酶链反应检测 14 种促血管生成 miRNA。通过定量冠状动脉造影评估 PCI 术后 12 个月时非靶病变的快速血管造影狭窄进展和靶病变的再狭窄。
结果
RASP 和再狭窄的发生率分别为 39.5%和 22.4%。与非 RASP 患者相比，RASP 患者中 let-7f、miR-19a、miR-19b-1、miR-92a、miR-126、miR-210 和 miR-296 的表达降低，其中 let-7f、miR-19a、miR-126、miR-210 和 miR-296 经多变量分析独立与较低的 RASP 发生率相关，随后 ROC 曲线显示这 5 种 miRNA 对预测 RASP 风险具有较高价值，曲线下面积（AUC）为 0.879（95%CI：0.841-0.917）。此外，与非再狭窄患者相比，再狭窄患者中 let-7f、miR-19a、miR-92a、miR-126、miR-130a 和 miR-210 的表达降低，其中 miR-19a、miR-126、miR-210 和 miR-378 经多变量分析独立与较低的再狭窄发生率相关，随后 ROC 曲线显示这 4 种 miRNA 对预测再狭窄风险具有较好价值，曲线下面积（AUC）为 0.776（95%CI：0.722-0.831）。
结论
在接受 DES 治疗的 CAD 患者中，循环 let-7f、miR-19a、miR-126、miR-210 和 miR-296 与降低 RASP 风险独立相关，而 miR-19a、miR-126、miR-210 和 miR-378 与降低再狭窄风险独立相关。
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