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Restoration of Ca2+-activated tension of CDTA-treated single skeletal muscle fibers by troponin C.

作者信息

Morimoto S, Fujiwara T, Ohtsuki I

机构信息

Department of Pharmacology, Faculty of Medicine, Kyushu University, Fukuoka.

出版信息

J Biochem. 1988 Dec;104(6):873-4. doi: 10.1093/oxfordjournals.jbchem.a122573.

DOI:10.1093/oxfordjournals.jbchem.a122573
PMID:3149638
Abstract

Single fibers from glycerinated rabbit psoas muscle were treated with a solution containing CDTA, a strong chelator of metal ions. The CDTA-treated fibers lost all of the troponin C and showed no Ca2+-activated tension development. The addition of troponin C restored the Ca2+-activated tension of CDTA-treated fibers. The tension-pCa relationship in the case of the CDTA-treated fibers reconstituted with troponin C was almost the same as that in the case of the same fibers before the CDTA treatment. These results are consistent with those of the previous study on the Ca2+-activated ATPase of CDTA-treated rabbit skeletal myofibrils.

摘要

相似文献

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Restoration of Ca2+-activated tension of CDTA-treated single skeletal muscle fibers by troponin C.
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