一种用纯化的荧光胺标记肽聚糖对溶菌酶进行的快速荧光检测法。
A rapid fluorimetric test for lysozyme with purified fluorescamine-labelled peptidoglycan.
作者信息
Moser I, Pittner F, Dworsky P
机构信息
Institut für Allgemeine Biochemie, Ludwig Boltzmann Forschungsstelle für Biochemie, Vienna, Austria.
出版信息
J Biochem Biophys Methods. 1988 Dec;17(4):249-52. doi: 10.1016/0165-022x(88)90048-6.
PMID:3149658
Abstract
A sensitive and rapid fluorometric lysozyme assay is described. It is based on the hydrolysis of fluorescamine-labelled peptidoglycan from Micrococcus luteus cell walls. Lysozyme levels as low as 0.1 microgram can be detected.
摘要
本文描述了一种灵敏且快速的荧光溶菌酶测定法。该方法基于对来自藤黄微球菌细胞壁的荧光胺标记的肽聚糖的水解。最低可检测到低至0.1微克的溶菌酶水平。
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