Watanabe M F, Oishi S, Harda K, Matsuura K, Kawai H, Suzuki M
Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Toxicon. 1988;26(11):1017-25. doi: 10.1016/0041-0101(88)90200-0.
Cyclic peptide toxins were analyzed for three Microcystis species (M. aeruginosa, M. viridis and M. wesenbergii) using an ODS-silica gel cartridge and high performance liquid chromatography with ODS-silica gel. On strain of M. aeruginosa contained a high amount of microcystin (cyanoginosin) YR and a lesser amount of LR. Three toxins, microcystin-RR, -YR and -LR, were detected in two strains of M. aeruginosa and four of M. viridis. The main component of the toxins of these strains was microcystin-RR. M. wesenbergii showed no peak in the area where the three toxins were obtained in other Microcytisis species by HPLC analysis. LD50 values of the purified toxins of YR and LR were similar, while a lower toxicity was estimated for RR. This explains the relatively weak toxicity of M. viridis whose main component is microcystin-RR.
使用ODS硅胶柱和配备ODS硅胶的高效液相色谱法，对三种微囊藻（铜绿微囊藻、绿色微囊藻和惠氏微囊藻）的环肽毒素进行了分析。一株铜绿微囊藻含有大量的微囊藻毒素（氰基微囊藻毒素）YR和少量的LR。在两株铜绿微囊藻和四株绿色微囊藻中检测到了三种毒素，即微囊藻毒素-RR、-YR和-LR。这些菌株毒素的主要成分是微囊藻毒素-RR。通过HPLC分析，惠氏微囊藻在其他微囊藻属物种获得三种毒素的区域未显示出峰。YR和LR纯化毒素的半数致死剂量（LD50）值相似，而RR的毒性估计较低。这解释了主要成分是微囊藻毒素-RR的绿色微囊藻毒性相对较弱的原因。
