Wang Qi, Sun Qian, Qi Renli, Wang Jing, Qiu Xiaoyu, Liu Zuohua, Huang Jinxiu
Chongqing Academy of Animal Sciences, Chongqing, China.
Key Laboratory of Pig Industry Sciences, Chongqing Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, China.
J Anim Physiol Anim Nutr (Berl). 2019 Nov;103(6):1908-1918. doi: 10.1111/jpn.13198. Epub 2019 Sep 9.
This study investigated the effect of Lactobacillus plantarum strain 299v on gut health in suckling piglets. Sixty newborn piglets were assigned to control and probiotic treatments, with three litters per treatment (ten piglets/litter). From days 1 to 20 of life, piglets were orally administered a placebo of 0.1% peptone or 1.0 × 10 CFU L. plantarum 299v daily. Six piglets per treatment were sacrificed on day 20, and intestinal tissues (including duodenum, jejunum, ileum and colon) and the intestinal contents from colon segments were collected. The results demonstrated that piglets treated with L. plantarum 299v had a lower diarrhoea incidence than the controls. L. plantarum 299v administration significantly increased the ratio of the villus height to the crypt depth in the jejunum and ileum, as well as the mRNA expression of jejunal occludin and ileal zonula occludens 1 (ZO-1). The L. plantarum treatment also increased the mRNA abundance of porcine β-defensin 2 (pBD2) and pBD3 in the jejunum and ileum and of toll-like receptors (TLRs), such as TLR2, TLR4, TLR6 and TLR9 in the ileum, and significantly upregulated the mRNA abundances of ileal pBD1 and colonic TLR4. Additionally, the L. plantarum 299v treatment significantly changed the structure of the colonic microbiota, as evidenced by the obvious increases in the relative abundances of the phyla Firmicutes and Actinobacteria and of the genus Lactobacillus. Our findings indicate that L. plantarum 299v facilitates the gut health of suckling piglets, probably by improving the intestinal morphology and intestinal barrier function and by modifying the structure of the gut microbiota.
本研究调查了植物乳杆菌299v菌株对哺乳仔猪肠道健康的影响。将60只新生仔猪分为对照组和益生菌处理组，每组3窝（每窝10只仔猪）。在仔猪出生后的第1至20天，每天给仔猪口服0.1%蛋白胨安慰剂或1.0×10CFU植物乳杆菌299v。在第20天，每组处死6只仔猪，收集肠道组织（包括十二指肠、空肠、回肠和结肠）以及结肠段的肠内容物。结果表明，用植物乳杆菌299v处理的仔猪腹泻发生率低于对照组。给予植物乳杆菌299v显著增加了空肠和回肠绒毛高度与隐窝深度的比值，以及空肠闭合蛋白和回肠紧密连接蛋白1（ZO-1）的mRNA表达。植物乳杆菌处理还增加了空肠和回肠中猪β-防御素2（pBD2）和pBD3以及回肠中Toll样受体（TLR）（如TLR2、TLR4、TLR6和TLR9）的mRNA丰度，并显著上调了回肠pBD1和结肠TLR4的mRNA丰度。此外，植物乳杆菌299v处理显著改变了结肠微生物群的结构，表现为厚壁菌门、放线菌门以及乳酸杆菌属的相对丰度明显增加。我们的研究结果表明，植物乳杆菌299v可能通过改善肠道形态和肠道屏障功能以及改变肠道微生物群的结构来促进哺乳仔猪的肠道健康。
