National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Road, Khlong Nueng, Khlong Luang, Pathumthani 12120, Thailand.
Ubon Ratchathani Rice Research Center, Rice Department, Ministry of Agriculture, Ubon Ratchathani 34000, Thailand.
Genomics. 2022 Jan;114(1):482-487. doi: 10.1016/j.ygeno.2019.09.003. Epub 2019 Sep 6.
Rice is an important crop that is consumed by approximately half of the world's population on a regular basis. Plant height is an important characteristic with shorter rice often having higher lodging resistance and better soil nutrient utilization allowing for lower fertilizer use. We used a Chromosome Segment Substitution Line (CSSL) population generated by introgressing segments of CT9993 and IR62266 into KDML 105. We identified height QTLs on chromosomes 1 and 4. We performed whole genome sequencing of the parental lines and found that IR62266 has the deletion in Gibberellin 20-oxidase 2 corresponding to the semi-dwarf 1 locus. However, short height on chromosome 1 came from CT9993 with no mutation in Gibberellin 20-oxidase 2, or any known height genes. The height QTL on chromosome 4 contains mutations in Peroxisome biogenesis protein 6, which has been linked to a reduced growth phenotype in A. thaliana, making this a good candidate height gene.
水稻是一种重要的作物，全球约有一半的人口经常食用。株高是一个重要的特征，较矮的水稻通常具有更高的抗倒伏性和更好的土壤养分利用效率，从而减少肥料的使用。我们利用 CT9993 和 IR62266 的片段导入 KDML 105 生成的染色体片段代换系（CSSL）群体，鉴定到了第 1 和第 4 染色体上的株高 QTL。我们对亲本进行了全基因组测序，发现 IR62266 在赤霉素 20-氧化酶 2 中发生了缺失，对应于半矮秆 1 位点。然而，第 1 染色体上的矮秆来自 CT9993，其赤霉素 20-氧化酶 2 没有突变，也没有任何已知的株高基因。第 4 染色体上的株高 QTL 含有过氧化物酶体生物发生蛋白 6 的突变，该蛋白与拟南芥的生长表型降低有关，使其成为一个很好的候选株高基因。
