Karawya E M
Department of Clinical Biochemistry, College of Medicine, Abha King Saud University, Saudi Arabia.
Ital J Biochem. 1988 Nov-Dec;37(6):365-79.
Restriction mapping of recombinant plasmids indicated the presence of poison sequence(s) in monkey mtDNA. These plasmids were constructed from a 5.2 K.b. BglII mtDNA fragment and pRSVneo or pdel9 as cloning vectors. The poison sequence(s) caused genetic rearrangement of the vectors' nucleotide sequences. Deletion of the suspected poison sequence(s) from the mtDNA fragment increased the transformation efficiency of the produced recombinant plasmids and conserved the vectors' original nucleotide sequences.
重组质粒的限制性图谱分析表明猴线粒体DNA中存在毒性序列。这些质粒由一个5.2千碱基对的BglII线粒体DNA片段与pRSVneo或pdel9作为克隆载体构建而成。毒性序列导致了载体核苷酸序列的基因重排。从线粒体DNA片段中删除可疑的毒性序列提高了所产生的重组质粒的转化效率，并保留了载体的原始核苷酸序列。
