College of Horticulture, Northwest A&F University, Taicheng Road No.3, Yangling, Shaanxi 712100, China.
Molecules. 2019 Sep 7;24(18):3263. doi: 10.3390/molecules24183263.
Garlic and formulations containing allicin are used widely as fungicides in modern agriculture. However, limited reports are available on the allelopathic mechanism of green garlic volatile organic compounds (VOCs) and its component allelochemicals. The aim of this study was to investigate VOCs of green garlic and their effect on scavenging of reactive oxygen species (ROS) in cucumber. In this study, green garlic VOCs were collected by HS-SPME, then analyzed by GS-MS. Their biological activity were verified by bioassays. The results showed that diallyl disulfide (DADS) is the main allelochemical of green garlic VOCs and the DADS content released from green garlic is approximately 0.08 mg/g. On this basis, the allelopathic effects of green garlic VOCs in vivo and 1 mmol/L DADS on scavenging of ROS in cucumber seedlings were further studied. Green garlic VOCs and DADS both reduce superoxide anion and increase the accumulation of hydrogen peroxide of cucumber seedlings. They can also regulate active antioxidant enzymes (SOD, CAT, POD), antioxidant substances (MDA, GSH and ASA) and genes (, , , , , ) responding to oxidative stress in cucumber seedlings.
大蒜和含有蒜素的制剂被广泛用作现代农业中的杀菌剂。然而，关于绿蒜挥发性有机化合物（VOCs）及其成分化感物质的化感作用机制的报道有限。本研究旨在研究绿蒜 VOCs 及其对黄瓜清除活性氧（ROS）的影响。在这项研究中，通过 HS-SPME 收集绿蒜 VOCs，然后通过 GS-MS 进行分析。通过生物测定验证它们的生物活性。结果表明，二烯丙基二硫（DADS）是绿蒜 VOCs 的主要化感物质，绿蒜释放的 DADS 含量约为 0.08mg/g。在此基础上，进一步研究了绿蒜 VOCs 在体内和 1mmol/L DADS 对黄瓜幼苗 ROS 清除的化感作用。绿蒜 VOCs 和 DADS 均能降低超氧阴离子自由基的产生，增加黄瓜幼苗中过氧化氢的积累。它们还可以调节黄瓜幼苗中响应氧化应激的活性抗氧化酶（SOD、CAT、POD）、抗氧化物质（MDA、GSH 和 ASA）和基因（，，，，，）。
