Cow ghee fortified ocular topical microemulsion; , , and evaluation.

Utility of cow ghee (CG) as permeation enhancer in development of topical ocular microemulsion (ME) for delivery of fluocinolone acetonide (FA) to posterior eye. For ME preparation, oil, surfactant and cosurfactant were screened based on solubility of FA. Pseudoternary phase diagrams were constructed to determine their ratios. The developed MEs were characterised for their physicochemical properties like size, polydispersity index, zeta potential, and stability etc. They were evaluated for permeation and irritation. pharmacokinetic studies were performed on Sprague dawley rats. Lauroglycol as oil, labrasol as surfactant and Transcutol as cosurfactant were selected. The optimised ratio of oil:surfactant:cosurfactant:water was 4:23:23:50. The developed FA loaded ME fortified with CG was characterised. study revealed higher permeation and non-irritancy. pharmacokinetic study showed retention of CG fortified ME in posterior rat eye. Present investigation established CG as permeation enhancer for ocular topical formulation.