Messina Concetta Maria, Gaglio Raimondo, Morghese Maria, Tolone Marco, Arena Rosaria, Moschetti Giancarlo, Santulli Andrea, Francesca Nicola, Settanni Luca
Laboratory of Marine biochemistry and ecotoxicology, Department of Earth and Marine Science DISTEM, University of Palermo, Via G. Barlotta 4, 91100 Trapani, Italy.
Department of Agricultural, Food and Forestry Science, University of Palermo, Viale delle Scienze 4, 90128 Palermo, Italy.
Foods. 2019 Sep 9;8(9):400. doi: 10.3390/foods8090400.
Microbiological, nutritional and bioactive properties of edible powders obtained from (house cricket) and (mealworm) were investigated. Except for the enterobacteria, viable bacteria were at a higher concentration in mealworm flour. The diversity evaluation carried out using MiSeq Illumina that mainly identified and in mealworm powder and members of the family in house cricket powder. Enterococci were identified and characterized for their safety characteristics in terms of the absence of antibiotic resistance and virulence. Both powders represent a good source of proteins and lipids. The fatty acid profile of mealworm powder was characterized by the predominance of the monounsaturated fatty acids and house cricket powder by saturated fatty acids. The enzymatic hydrolysis produced the best results in terms of percentage of degree of hydrolysis with the enzyme Alcalase, and these data were confirmed by SDS-PAGE electrophoresis. Furthermore, the results showed that the protein hydrolysate of these powders produces a significant antioxidant power.
对从家蟋蟀和黄粉虫获得的可食用粉末的微生物学、营养和生物活性特性进行了研究。除肠杆菌外,黄粉虫粉中活菌浓度更高。使用MiSeq Illumina进行的多样性评估主要在黄粉虫粉中鉴定出芽孢杆菌属和葡萄球菌属,在家蟋蟀粉中鉴定出肠杆菌科成员。对肠球菌进行了鉴定,并根据其无抗生素抗性和毒力的安全性特征进行了表征。两种粉末都是蛋白质和脂质的良好来源。黄粉虫粉的脂肪酸谱以单不饱和脂肪酸为主,家蟋蟀粉以饱和脂肪酸为主。就水解度百分比而言,用碱性蛋白酶进行酶水解产生了最佳结果,这些数据通过SDS-PAGE电泳得到证实。此外,结果表明,这些粉末的蛋白质水解产物具有显著的抗氧化能力。