Unité de Chimie Environnementale et Interactions sur le Vivant) (UCEIV), EA 4492, SFR Condorcet FR CNRS 3417, Université du Littoral Côte d'Opale, 50 rue Ferdinand Buisson, 62228, Calais, France.
Paris Institute of Ecology and Environnemental Sciences (IEES-Paris), UMR 7518 (CNRS- SU-INRA-UPEC- Paris Diderot-IRD), Université Paris-Est Créteil, 60 avenue du Général de Gaulle, 94010, Créteil Cedex, France.
Mycorrhiza. 2019 Oct;29(5):475-487. doi: 10.1007/s00572-019-00914-1. Epub 2019 Sep 7.
Arbuscular mycorrhizal fungi (AMF) play a central role in rhizosphere functioning as they interact with both plants and soil microbial communities. The conditions in which AMF modify plant physiology and microbial communities in the rhizosphere are still poorly understood. In the present study, four different plant species, (clover, alfalfa, ryegrass, tall fescue) were cultivated in either sterilized (γ ray) or non-sterilized soil and either inoculated with a commercial AMF (Glomus LPA Val 1.) or not. After 20 weeks of cultivation, the mycorrhizal rate and shoot and root biomasses were measured. The abundance and composition of bacteria, archaea, and fungi were analyzed, respectively, by quantitative PCR (qPCR) and fingerprinting techniques. Whilst sterilization did not change the AMF capacity to modify plant biomass, significant changes in microbial communities were observed, depending on the taxon and the associated plant. AMF inoculation decreases both bacterial and archaeal abundance and diversity, with a greatest extent in sterilized samples. These results also show that AMF exert different selections on soil microbial communities according to the plant species they are associated with. This study suggests that the initial abundance and diversity of rhizosphere microbial communities should be considered when introducing AMF to cultures.
丛枝菌根真菌（AMF）在根际发挥着核心作用，因为它们与植物和土壤微生物群落相互作用。然而，AMF 在根际中改变植物生理学和微生物群落的条件仍知之甚少。在本研究中，我们将四种不同的植物物种（三叶草、紫花苜蓿、黑麦草、高羊茅）分别种植在经过灭菌（γ 射线）或未灭菌的土壤中，并分别接种或不接种商业 AMF（Glomus LPA Val 1.）。经过 20 周的培养，测量了菌根率、地上部和根生物量。通过定量 PCR（qPCR）和指纹图谱技术，分别分析了细菌、古菌和真菌的丰度和组成。虽然灭菌不改变 AMF 改变植物生物量的能力，但观察到微生物群落发生了显著变化，具体取决于分类群和相关植物。AMF 接种减少了细菌和古菌的丰度和多样性，在灭菌样本中程度最大。这些结果还表明，AMF 根据它们所关联的植物物种对土壤微生物群落施加不同的选择。本研究表明，在引入 AMF 到培养物时，应该考虑根际微生物群落的初始丰度和多样性。
