Bandyopadhyay R, Basu M K
Biomembrane Division, Indian Institute of Chemical Biology, Calcutta.
Biochimie. 1988 Dec;70(12):1841-7. doi: 10.1016/0300-9084(88)90046-6.
Total phospholipids were extracted from the heart, hepatopancreas, and hemolymph of the Indian horseshoe crab Carcinoscorpius rotundicauda by the conventional method. Characteristic group reaction and 2-dimensional thin-layer chromatography on silica gel were used for identification of different phospholipids. The phospholipid profile obtained from hemolymph and 2 major organs are comparable and show phosphatidyl choline (PC) and phosphatidyl ethanolamine to be the major phospholipids. A phospholipid has been consistently detected migrating immediately below the PC in the thin-layer chromatogram of lipids extracted from the hepatopancreas. When mixed methyl esters of this slower moving PC are resolved on a silica gel plate ran in hexane ether:acetic acid 80:20:1, with appropriate controls, an additional spot is seen just below the normal methyl ester, indicating a difference between the fatty acid compositions of 2 PC (e.g., regular and slower). The slower mixed methyl esters were found to comprise mainly the 4 saturated fatty acids: lauric, myristic, palmitic, and stearic. The slow moving PC seems to consist mainly of molecular species with the above-mentioned saturated fatty acids at both Sn 1 and Sn 2 positions.
采用常规方法从印度鲎圆尾蝎鲎的心脏、肝胰腺和血淋巴中提取总磷脂。利用特征基团反应和硅胶二维薄层色谱法对不同磷脂进行鉴定。从血淋巴和两个主要器官获得的磷脂图谱具有可比性，表明磷脂酰胆碱（PC）和磷脂酰乙醇胺是主要的磷脂。在从肝胰腺提取的脂质的薄层色谱图中，始终检测到一种磷脂在PC下方紧邻处迁移。当这种迁移较慢的PC的混合甲酯在以己烷醚：乙酸80:20:1展开的硅胶板上进行分离，并设置适当对照时，在正常甲酯下方会出现一个额外的斑点，表明两种PC的脂肪酸组成存在差异（例如，正常的和迁移较慢的）。发现迁移较慢的混合甲酯主要由4种饱和脂肪酸组成：月桂酸、肉豆蔻酸、棕榈酸和硬脂酸。迁移较慢的PC似乎主要由在Sn 1和Sn 2位置都含有上述饱和脂肪酸的分子种类组成。
