Berkoz M
Yuzuncu Yil University, Faculty of Pharmacy - Department of Biochemistry, Van, Turkey.
Acta Endocrinol (Buchar). 2019 Apr-Jun;15(2):165-172. doi: 10.4183/aeb.2019.165.
The inhibition of adipocyte differentiation has a significant role on the prevention of obesity and obesity-associated complications.
In this study, we aimed to detect whether hyperoside is able to inhibit the conversion of pre-adiposits into mature adiposits.
3T3-L1 pre-adipocytes were stimulated so as to differentiate into mature adipocytes. Hyperoside in non-cytotoxic concentrations (1, 2, 5, and 10 µM) were separately applied to differentiated 3T3-L1 cells. Oil red O staining was performed and triacylglycerol contents were measured. Furthermore, gene and protein expressions of transcription factors, adipogenic genes and adipokines were examined in order to investigate the effect of hyperoside on adipocyte differentiation.
Hyperoside in high concentrations significantly suppressed the adipogenic process by inhibiting the expression of transcription factors and adipogenic genes and reducing lipid accumulation in adipocytes (p<0.05). Low doses of hyperoside are able to inhibit adipogenesis, but higher doses are needed to reduce fat accumulation in mature adipocytes. In the case of maturing preadipocytes, 5 μM of hyperoside exerts its antiadipogenic effect at the early stages of adipogenesis, whereas 10 μM of hyperoside acts at the later stages (p<0.05).
These results suggest that hyperoside has a beneficial effect on the prevention and treatment of obesity.
抑制脂肪细胞分化对预防肥胖及肥胖相关并发症具有重要作用。
在本研究中，我们旨在检测金丝桃苷是否能够抑制前脂肪细胞向成熟脂肪细胞的转化。
刺激3T3-L1前脂肪细胞使其分化为成熟脂肪细胞。将无细胞毒性浓度（1、2、5和10 μM）的金丝桃苷分别应用于分化后的3T3-L1细胞。进行油红O染色并测量三酰甘油含量。此外，检测转录因子、脂肪生成相关基因和脂肪因子的基因及蛋白表达，以研究金丝桃苷对脂肪细胞分化的影响。
高浓度的金丝桃苷通过抑制转录因子和脂肪生成相关基因的表达以及减少脂肪细胞中的脂质积累，显著抑制脂肪生成过程（p<0.05）。低剂量的金丝桃苷能够抑制脂肪生成，但需要更高剂量才能减少成熟脂肪细胞中的脂肪积累。对于正在成熟的前脂肪细胞，5 μM的金丝桃苷在脂肪生成的早期阶段发挥其抗脂肪生成作用，而10 μM的金丝桃苷在后期发挥作用（p<0.05）。
这些结果表明金丝桃苷对肥胖的预防和治疗具有有益作用。
