在糖尿病大鼠心脏中，联合进行的自愿运动和睾酮显著降低了氧化应激。
OXIDATIVE STRESS IS MARKEDLY REDUCED BY COMBINED VOLUNTARY EXERCISE AND TESTOSTERONE IN THE HEART OF DIABETIC RATS.
作者信息
Chodari L, Smailnejad S, Fallahi M, Khalaji N, Ghorbanzadeh V
机构信息
Department of Physiology, Urmia University of Medical Sciences, Urmia, Iran.
Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran.
出版信息
Acta Endocrinol (Buchar). 2019 Apr-Jun;15(2):173-181. doi: 10.4183/aeb.2019.173.
OBJECTIVE
Cardiovascular disorders in diabetes condition arise from increased oxidative stress. Both regular mild exercise and testosterone influence on body's antioxidant system in diabetes. In this study, we evaluated treatment of testosterone and voluntary exercise, alone or together on oxidative stress in the heart and blood of diabetic rats.
METHODS
Type 1 diabetes was induced by intraperitoneal injection of 50 mg/kg of streptozotocin in rats. Sixty three rats have been divided into eight groups as follows: Diabetes, diabetes+ testosterone, diabetes+ exercise, diabetes+ testosterone+ exercise, diabetes+ castration, diabetes+ castration+ testosterone, Diabetes+ castration+ exercise, Diabetes+ castration+ exercise+ testosterone. Type 1 diabetes was induced by intraperitoneal injection of 50 mg/kg of streptozotocin in the male Wistar rats and after a week, castration was performed. After 42 days of treatment with testosterone (2 mg/kg/day) or voluntary exercise alone or in combination, SOD, GPX and CAT activities and MDA levels were measured in the blood and heart tissue samples in the groups of study. In the end of study, SOD, GPX and CAT activities and MDA levels were measured in blood and heart tissue samples in the groups of study.
RESULTS
SOD, GPX and CAT activities significantly (p<0.05) increased in groups that treated either testosterone or exercise and MDA level significantly (p<0.01) decreased in the blood and heart tissue of diabetic and castrated diabetic rats. Simultaneously, treatment with testosterone and exercise had a synergistic effect on antioxidant enzymes level in diabetic and diabetic castrated rats. In the castrated animals with diabetes, SOD, GPX and CAT activities significantly decreased (p<0.05) and MDA levels significantly increased (p<0.05) in blood and heart tissue.
CONCLUSION
Voluntary exercise and testosterone alone or together heightened body's antioxidant system and were able to reduce the MDA levels in blood and heart of diabetic and castrated diabetic rats.
目的
糖尿病状态下的心血管疾病源于氧化应激增加。规律的轻度运动和睾酮均会影响糖尿病患者体内的抗氧化系统。在本研究中，我们评估了单独或联合使用睾酮和自主运动对糖尿病大鼠心脏和血液氧化应激的治疗作用。
方法
通过腹腔注射50mg/kg链脲佐菌素诱导大鼠患1型糖尿病。63只大鼠被分为八组，如下：糖尿病组、糖尿病+睾酮组、糖尿病+运动组、糖尿病+睾酮+运动组、糖尿病+去势组、糖尿病+去势+睾酮组、糖尿病+去势+运动组、糖尿病+去势+运动+睾酮组。通过腹腔注射50mg/kg链脲佐菌素诱导雄性Wistar大鼠患1型糖尿病，一周后进行去势手术。在用睾酮（2mg/kg/天）单独或联合自主运动治疗42天后，测定研究组血液和心脏组织样本中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）和过氧化氢酶（CAT）活性以及丙二醛（MDA）水平。在研究结束时，测定研究组血液和心脏组织样本中的SOD、GPX和CAT活性以及MDA水平。
结果
在接受睾酮或运动治疗的组中，SOD、GPX和CAT活性显著（p<0.05）升高，糖尿病和去势糖尿病大鼠的血液和心脏组织中MDA水平显著（p<0.01）降低。同时，睾酮和运动联合治疗对糖尿病和去势糖尿病大鼠的抗氧化酶水平具有协同作用。在去势的糖尿病动物中，血液和心脏组织中的SOD、GPX和CAT活性显著降低（p<0.05），MDA水平显著升高（p<0.05）。
结论
单独或联合使用自主运动和睾酮均可增强机体抗氧化系统，并能够降低糖尿病和去势糖尿病大鼠血液和心脏中的MDA水平。
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