Volume-CLEM：一种用于三维共聚焦和电子显微镜相关的方法。

Volume-CLEM: a method for correlative light and electron microscopy in three dimensions.

机构信息

Institute of Functional and Applied Anatomy, Hannover Medical School, Hanover, Germany.

Research Core Unit Electron Microscopy, Hannover Medical School, Hanover, Germany.

出版信息

Am J Physiol Lung Cell Mol Physiol. 2019 Dec 1;317(6):L778-L784. doi: 10.1152/ajplung.00333.2019. Epub 2019 Sep 11.

DOI:10.1152/ajplung.00333.2019

PMID:31508976

Abstract

Generation of three-dimensional (3D) data sets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative LM and electron microscopy (EM) from 3D LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.

摘要

从通过光学显微镜（LM）成像的组织的连续切片中生成三维（3D）数据集，允许通过形态或荧光标记来识别罕见结构。在这里，我们展示了一种从 3D LM 到 3D EM 的相关 LM 和电子显微镜（EM）工作流程，两种方法均连续使用相同的切片材料。这种新方法在常规 EM 实验室中易于重现，适用于广泛的器官和研究问题。