Plant Gene Expression Lab, CSIR-National Botanical Research Institute (Council of Scientific and Industrial Research), Lucknow, 226001, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Plant Gene Expression Lab, CSIR-National Botanical Research Institute (Council of Scientific and Industrial Research), Lucknow, 226001, India.
Plant Sci. 2019 Nov;288:110242. doi: 10.1016/j.plantsci.2019.110242. Epub 2019 Aug 30.
Abscission is a developmental process that leads to shedding of organs not needed by the plant. Apart from wall hydrolysis, the cells of the abscission zone (AZ) are also believed to undergo programmed cell death (PCD). We show that ethylene-induced petal abscission in Rosa bourboniana is accompanied with the activation of RbPCD1 (PROGRAMMED CELL DEATH LIKE 1) encoding a protein of 78 amino acids. Its expression increases during natural and ethylene-induced petal abscission. Its transcription in most tissues is up-regulated by ethylene. RbPCD1 shows similarity to the N-terminal domain of animal PDCD4 (PROGRAMMED CELL DEATH PROTEIN 4) proteins that are activated during apoptosis and function as transcriptional and translational repressors. RbPCD1 resides in the nucleus and cytoplasm and acts as a transcriptional repressor. Constitutive expression of RbPCD1 in transgenic Arabidopsis is seedling lethal. Heat-induced expression of RbPCD1 under the soybean heat-shock promoter affects leaf function, inflorescence development, silique formation, seed yield and reduces survival. Nuclear localization of RbPCD1 is necessary for manifestation of its effects. RbPCD1 may be necessary to mediate some of the ethylene-induced changes during abscission and senescence in specific tissues.
离区是导致植物不需要的器官脱落的发育过程。除细胞壁水解外，离区（AZ）的细胞也被认为经历程序性细胞死亡（PCD）。我们表明，Rosa bourboniana 中乙烯诱导的花瓣脱落伴随着 RbPCD1（程序性细胞死亡样 1）的激活，该基因编码一个 78 个氨基酸的蛋白质。其表达在自然和乙烯诱导的花瓣脱落过程中增加。乙烯在大多数组织中上调其转录。RbPCD1 与动物 PDCD4（程序性细胞死亡蛋白 4）的 N 端结构域相似，该结构域在细胞凋亡过程中被激活，作为转录和翻译抑制剂发挥作用。RbPCD1 存在于细胞核和细胞质中，作为转录抑制剂发挥作用。RbPCD1 在转基因拟南芥中的组成性表达导致幼苗致死。在大豆热休克启动子下热诱导的 RbPCD1 表达会影响叶片功能、花序发育、蒴果形成、种子产量并降低存活率。RbPCD1 的核定位对于其效应的表现是必要的。RbPCD1 可能需要在特定组织中介导乙烯诱导的脱落和衰老过程中的一些变化。
