College of Veterinary Medicine, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Dalian Lvshun Longtou Animal Health Supervision Institute, Dalian 116041, China.
Int J Biol Macromol. 2019 Dec 1;141:1220-1227. doi: 10.1016/j.ijbiomac.2019.09.086. Epub 2019 Sep 12.
This study aimed to investigate the potential protective effects of Platycodon grandiflorus polysaccharide (PGPS) on carbonyl cyanide 3-chlorophenylhydrazone (CCCP)-induced mitochondrial apoptosis in 3D4/21 cells. Apoptosis-related indicators such as cell viability, apoptosis rate, mitochondrial membrane potential (MMP), and apoptosis-related protein were examined. Results indicated that PGPS can inhibit CCCP-induced cell damage, with cell-survival rate reaching 81% and apoptotic rate decreasing to 23%. Nuclear deformation was also significantly reduced in the PGPS group, and changes in MMP were inhibited by PGPS. Further analyses showed that the protein expression of Caspase-9 and Bcl-2 increased and the expression of cleaved Caspase-3 decreased, indicating that PGPS significantly inhibited the CCCP-induced change in apoptotic protein expression. All these results suggested that PGPS can antagonize 3D4/21 cell apoptosis by restoring MMP, protecting the integrity of nuclear morphology, and increasing Bcl-2 expression.
本研究旨在探讨桔梗多糖（PGPS）对 3D4/21 细胞羰基氰化物 3-氯苯腙（CCCP）诱导的线粒体凋亡的潜在保护作用。检测了细胞活力、凋亡率、线粒体膜电位（MMP）和凋亡相关蛋白等凋亡相关指标。结果表明，PGPS 可抑制 CCCP 诱导的细胞损伤，细胞存活率达到 81%，凋亡率降低至 23%。PGPS 组核变形也明显减少，MMP 的变化受到 PGPS 的抑制。进一步分析表明，PGPS 显著抑制 CCCP 诱导的凋亡蛋白表达变化，使 Caspase-9 和 Bcl-2 的蛋白表达增加，而 cleaved Caspase-3 的表达减少。这些结果表明，PGPS 可以通过恢复 MMP、保护核形态的完整性和增加 Bcl-2 表达来拮抗 3D4/21 细胞凋亡。
