Zheng Pimiao, Fan Wentao, Wang Shenghua, Hao Pan, Wang Yang, Wan Huiyu, Hao Zhihui, Liu Jianzhu, Zhao Xiaona
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
Agricultural Bio-pharmaceutical Lab. Qingdao Agricultural University, Qingdao 266000, PR China.
Int J Biol Macromol. 2017 Mar;96:775-785. doi: 10.1016/j.ijbiomac.2016.12.077. Epub 2017 Jan 2.
Polysaccharides were isolated from Platycodon grandiflorus (Jacq.) A.DC. (PG) and the effects of three polysaccharides (PGPS, PGPS, PGPS) on their immunological activities were studied. The structure identification of PGPSs was assessed using physicochemical and spectral methods. Results showed that PGPS(2.67×10Da) compared to PGPS(1.01×10Da) and PGPS(1.12×10Da) has relatively higher average molecular weight(Mw) at the first peak with a narrower molecular weight distribution and all consisted of glucose, mannose, arabinose, galactose, xylose and rhamnose in different mass percentages. PGPS and PGPS linked mainly by 1,3-and 1,6-β-d-Galp residues. The immunological efficacy of PGPSs was performed on chicken peritoneal macrophages. Results showed that PGPS significantly increased phagocytic rates, proliferation and NO production, stimulated macrophages to produce cytokines, including TNF-α, IL-1β and IL-6 as well as stimulated macrophages to express the maturation markers CD80 and CD86. These findings suggest that PGPS exerted significant immunological activity and might be associated with special characters.
从桔梗（Platycodon grandiflorus (Jacq.) A.DC.，PG）中分离出多糖，并研究了三种多糖（PGPS、PGPS、PGPS）的免疫活性。采用物理化学和光谱方法对PGPSs进行结构鉴定。结果表明，与PGPS（1.01×10Da）和PGPS（1.12×10Da）相比，PGPS（2.67×10Da）在第一个峰处具有相对较高的平均分子量（Mw），分子量分布较窄，且均由不同质量百分比的葡萄糖、甘露糖、阿拉伯糖、半乳糖、木糖和鼠李糖组成。PGPS和PGPS主要由1,3-和1,6-β-D-半乳糖残基连接。在鸡腹膜巨噬细胞上进行了PGPSs的免疫功效研究。结果表明，PGPS显著提高吞噬率、增殖和NO产生，刺激巨噬细胞产生细胞因子，包括TNF-α、IL-1β和IL-6，以及刺激巨噬细胞表达成熟标志物CD80和CD86。这些发现表明，PGPS具有显著的免疫活性，可能与特殊特性有关。
