Langweiler Marc
, Asheville, NC, USA.
Methods Mol Biol. 2019;2032:69-79. doi: 10.1007/978-1-4939-9650-6_4.
Antibody reagents that are used for flow cytometry immunophenotyping have traditionally been prepared by combining individual liquid antibody conjugates into mixtures. These cocktails have limited shelf-life, and their preparation is time-consuming and prone to laboratory error. Manufacturers of these reagents, in collaboration with several clinical and research centers, have made advances in constructing dried antibody cocktails which have addressed many of the problems inherent in preparing the liquid cocktails on the lab bench. This chapter discusses methods for the use of dried reagents.
传统上，用于流式细胞术免疫表型分析的抗体试剂是通过将各个液体抗体偶联物混合而成的。这些混合物的保质期有限，而且其制备过程耗时且容易出现实验室误差。这些试剂的制造商与多个临床和研究中心合作，在构建干燥抗体混合物方面取得了进展，解决了在实验室工作台上制备液体混合物所固有的许多问题。本章讨论使用干燥试剂的方法。
