循环内皮细胞和内皮微粒的免疫表型分析
Immunophenotyping of Circulating Endothelial Cells and Endothelial Microparticles.
作者信息
Wanner Nicholas, Asosingh Kewal
机构信息
Department of Inflammation and Immunity, Cleveland Clinic, Lerner Research Institute, Cleveland, OH, USA.
出版信息
Methods Mol Biol. 2019;2032:203-211. doi: 10.1007/978-1-4939-9650-6_12.
PMID:31522421
Abstract
Flow-cytometric detection of circulating endothelial cells and endothelial microparticles is an essential tool in studies of vascular diseases. Here we describe the principles and detailed methods for human blood sample processing, storage, labeling, and gating of circulating endothelial elements.
摘要
流式细胞术检测循环内皮细胞和内皮微粒是血管疾病研究中的一项重要工具。在此，我们描述了人体血液样本处理、储存、标记及循环内皮细胞成分设门的原理和详细方法。
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