Department of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.
Flow Cytometry Core Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.
Cytometry A. 2021 Mar;99(3):257-264. doi: 10.1002/cyto.a.24293. Epub 2020 Dec 23.
In vascular research, clinical samples and samples from animal models are often used together to foster translation of preclinical findings to humans. General concepts of endothelia and murine-specific endothelial phenotypes were discussed in part 1 of this two part series. Here, in part 2, we present a comprehensive overview of human-specific endothelial phenotypes. Pan-endothelial cell markers, organ specific endothelial antigens, and flow cytometric immunophenotyping of blood-borne endothelial cells are reviewed.
在血管研究中，临床样本和动物模型样本通常一起使用，以促进临床前发现向人类的转化。在本系列两部分的第一部分中，讨论了内皮的一般概念和鼠特异性内皮表型。在这里，第二部分中，我们全面概述了人类特异性内皮表型。综述了泛内皮细胞标志物、器官特异性内皮抗原和血流细胞术免疫表型分析。
