DiGiuseppe Joseph A, Cardinali Jolene L
Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT, USA.
Methods Mol Biol. 2019;2032:297-310. doi: 10.1007/978-1-4939-9650-6_16.
Immunophenotyping by flow cytometry is an important component in the diagnostic evaluation of patients with acute lymphoblastic leukemia. This technique further permits the detection of minimal residual disease after therapy, a robust prognostic factor that may guide individualized treatment. We describe here laboratory methods for both the initial characterization of lymphoblasts at diagnosis, and the detection of rare leukemic lymphoblasts after treatment. In addition to antibody combinations suitable for diagnosis and detection of minimal residual disease, we describe procedures for peripheral blood and bone marrow sample preparation, procedures for labeling of cell-surface and intracellular proteins with fluorochrome-conjugated antibodies, and approaches to analysis of immunophenotypic data.
流式细胞术免疫表型分析是急性淋巴细胞白血病患者诊断评估的重要组成部分。该技术还能在治疗后检测微小残留病，这是一个强大的预后因素，可指导个体化治疗。我们在此描述诊断时原始淋巴细胞初始特征分析以及治疗后罕见白血病原始淋巴细胞检测的实验室方法。除了适用于微小残留病诊断和检测的抗体组合外，我们还描述了外周血和骨髓样本制备程序、用荧光染料偶联抗体标记细胞表面和细胞内蛋白的程序以及免疫表型数据分析方法。
