Aroni K, Fotiou G, Liossi A, Patsouris E, Agapitos E
Odontostomatol Proodos. 1988 Oct;42(5):371-5.
Lysozyme and lactoferrin, substances of the non-specific defense system of the salivary glands, were studied in normal human parotid glands of neonates and adults using the immunoperoxidase method. To our knowledge, the immunohistochemical detection and distribution of lysozyme and lactoferrin in neonate parotid glands have not been previously reported. In neonate parotid glands, a monotonous positive reaction for lysozyme was found in the acini, in the intercalated ducts and in a few cells of large ducts. On the contrary, lysozyme was observed mainly in the intercalated ducts of the adult parotid glands. Three staining patterns for lactoferrin were found in neonate parotid glands. The first pattern was identical to that of lysozyme in neonates, the second was similar to that of lactoferrin in adults, and in the third extremely few acinar and intercalated duct cells were positive. In adult parotid glands, lactoferrin was detected in groups of acini and intercalated ducts and rarely striated duct cells. In adult parotid glands, our findings are discussed in correlation with those of other investigators. The results of our study indicate that lysozyme and lactoferrin have an important role in the defense mechanism of neonate parotid gland. There is also a distinct immunohistochemical difference between neonate and adult parotid gland. Since it is known that there is a morphological differentiation of the parotid gland postnatally, it is presumably suggested that an immunohistochemical differentiation also occurs.
采用免疫过氧化物酶法，对新生儿和成人正常腮腺中唾液腺非特异性防御系统的物质——溶菌酶和乳铁蛋白进行了研究。据我们所知，此前尚未有关于新生儿腮腺中溶菌酶和乳铁蛋白免疫组化检测及分布的报道。在新生儿腮腺中，在腺泡、闰管以及少数大导管细胞中发现了溶菌酶单一的阳性反应。相反，溶菌酶主要在成人腮腺的闰管中观察到。在新生儿腮腺中发现了乳铁蛋白的三种染色模式。第一种模式与新生儿溶菌酶的模式相同，第二种与成人乳铁蛋白的模式相似，第三种模式中极少的腺泡和闰管细胞呈阳性。在成人腮腺中，在腺泡群和闰管中检测到乳铁蛋白，而在纹状管细胞中很少见。在成人腮腺中，我们将研究结果与其他研究者的结果进行了相关性讨论。我们的研究结果表明，溶菌酶和乳铁蛋白在新生儿腮腺的防御机制中起重要作用。新生儿和成人腮腺之间也存在明显的免疫组化差异。由于已知腮腺在出生后存在形态学分化，推测免疫组化分化也会发生。
