Hozumi M, Umezawa T, Takenaga K, Ohno T, Shikita M, Yamane I
Cancer Res. 1979 Dec;39(12):5127-31.
A clone, YS-T22, of cells from Yoshida sarcoma cell line, YSSF-212T, grown in "serum-free" culture medium produced factors stimulating differentiation of mouse myeloid leukemia cells (M1) to macrophages and granulocytes. The formation of macrophages and granulocytes was accompanied by induction of phagocytosis, locomotive activity, and lysosomal enzyme activities. The rates of induction of these differentiated phenotypes were proportional to the concentration of the factor added and the period of treatment. The factor stimulating differentiation of M1 cells was a heat-labile, nondialyzable proteinaceous substance that was inactivated by trypsin but not by ribonuclease or glycosidases. On diethylaminoethyl cellulose chromatography, the factor stimulating differentiation of M1 cells from conditioned medium of YS-T22 cells was eluted in various fractions with or without activity of the colony-stimulating factor.
从吉田肉瘤细胞系YSSF - 212T中获取的一个克隆细胞YS - T22，在“无血清”培养基中培养，产生了刺激小鼠髓性白血病细胞（M1）分化为巨噬细胞和粒细胞的因子。巨噬细胞和粒细胞的形成伴随着吞噬作用、运动活性和溶酶体酶活性的诱导。这些分化表型的诱导速率与添加因子的浓度和处理时间成正比。刺激M1细胞分化的因子是一种热不稳定、不可透析的蛋白质物质，可被胰蛋白酶灭活，但不能被核糖核酸酶或糖苷酶灭活。在二乙氨基乙基纤维素色谱上，从YS - T22细胞条件培养基中刺激M1细胞分化的因子在不同级分中被洗脱，这些级分有或没有集落刺激因子的活性
