Akmal Muslim, Gholib Gholib, Rinidar Rinidar, Fitriani Fitriani, Helmi T Zahrial, Sugito Sugito, Isa M, Nurliana Nurliana, Wahyuni Sri, Dasrul Dasrul, Yaman M Aman
Laboratory of Histology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
Laboratory of Physiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Aceh, Indonesia.
Vet World. 2019 Jul;12(7):1101-1107. doi: 10.14202/vetworld.2019.1101-1107. Epub 2019 Jul 25.
Testis and epididymis are male reproductive organs that play an important role in spermatogenesis These two organs are rich in the content of hormones and other molecules needed in the process of spermatogenesis which affect the quality of the spermatozoa. The objective of this study was to examine the effect of the administration of epididymis and testicular extracts and their combination on testosterone, pituitary adenylate cyclase-activating polypeptide (PACAP), and protamine 1 (PRM1) concentrations in the serum of male chicken.
Twenty male chickens (broiler strain Cp707), aged 3 weeks and weighing 800-1000 g, were randomly divided into four different groups including a control group (T0) = injected with 1 ml normal saline and treatment groups: T1 = injected with 1 ml epididymis extract, T2 = injected with 1 ml testicular extract, and T3 = injected with a combination of 1 ml epididymis + 1 ml testicular extract. The experiment was conducted for 13 days and at the end of the study (day 14), the chickens were sacrificed to obtain the serum. Furthermore, the concentrations of testosterone, PACAP, and PRM1 were then measured by using an enzyme-linked immunosorbent assay technique.
The concentrations of PACAP and PRM1 did not show a significant difference between treatment groups (T1, T2, and T3) and control group (T0) (p>0.05). However, the concentration of testosterone showed a significantly higher difference in a group injected with a combination of 1 ml epididymis and 1 ml testicular extracts (T3) compared to the control group (T0) (p<0.05).
The administration of epididymis and testicular extracts and their combination did not affect the increase of PACAP and PRM1 concentration. However, a combination of these extracts significantly affects the increase of testosterone concentration in the serum of male chicken.
睾丸和附睾是男性生殖器官，在精子发生过程中发挥着重要作用。这两个器官富含精子发生过程中所需的激素和其他分子，这些物质会影响精子的质量。本研究的目的是检测附睾和睾丸提取物及其组合给药对雄性鸡血清中睾酮、垂体腺苷酸环化酶激活多肽（PACAP）和鱼精蛋白1（PRM1）浓度的影响。
20只3周龄、体重800 - 1000克的雄性鸡（肉鸡品系Cp707）被随机分为四个不同组，包括对照组（T0）=注射1毫升生理盐水，治疗组：T1 =注射1毫升附睾提取物，T2 =注射1毫升睾丸提取物，T3 =注射1毫升附睾提取物 + 1毫升睾丸提取物的组合。实验进行13天，在研究结束时（第14天），处死鸡以获取血清。此外，然后使用酶联免疫吸附测定技术测量睾酮、PACAP和PRM1的浓度。
治疗组（T1、T2和T3）与对照组（T0）之间，PACAP和PRM1的浓度没有显示出显著差异（p>0.05）。然而，与对照组（T0）相比，注射1毫升附睾提取物和1毫升睾丸提取物组合的组（T3）中睾酮浓度显示出显著更高的差异（p<0.05）。
附睾和睾丸提取物及其组合给药不会影响PACAP和PRM1浓度的增加。然而，这些提取物的组合显著影响雄性鸡血清中睾酮浓度的增加。
