Key Laboratory of Textile Science and Technology of Ministry of Education and College of Textiles, Donghua University, 2999 North Renmin Road, Shanghai 201620, People's Republic of China.
Biomed Mater. 2019 Oct 8;14(6):065012. doi: 10.1088/1748-605X/ab452b.
Surface modification by long-term active component is essential for biocompatible polymers-based vascular grafts to prevent thrombus formation and reduce intimal hyperplasia. In this study, a simple approach was developed to immobilize bioactive heparin to the surface of ε-polycaprolactone (PCL) grafts through a two-step strategy combining covalent grafting and layer by layer assembly of polyelectrolytes. The performance of heparinized PCL was evaluated in vitro, including the release behavior of heparin, anticoagulation and different types of cells adhesion characteristic. A sustained-release of heparin was achieved by this immobilization strategy. Surface remaining heparin was up to 1.10 μg cm on the modified PCL after release in vitro for 30 d. Specifically, the heparinized PCL has the long-term ability to prevent adhesion of blood cells and thrombus formation, and significantly inhibit the adhesion of smooth muscle cells. The two-step strategy provides a simple and general route to incorporate heparin on PCL graft surface. The surface heparinized PCL demonstrated in this work can be a useful material platform for biodegradable vascular stent graft.
通过长期的活性成分表面修饰对于基于生物相容聚合物的血管移植物至关重要，可防止血栓形成并减少内膜增生。在这项研究中，开发了一种简单的方法，通过两步策略将生物活性肝素固定在ε-聚己内酯（PCL）移植物的表面，该策略结合了共价接枝和聚电解质的层层组装。通过体外实验评估了肝素化 PCL 的性能，包括肝素的释放行为、抗凝和不同类型细胞的黏附特性。通过这种固定化策略实现了肝素的持续释放。在体外释放 30 天后，改性 PCL 表面上的肝素残留量高达 1.10μgcm。具体来说，肝素化 PCL 具有长期防止血细胞黏附和血栓形成的能力，并能显著抑制平滑肌细胞的黏附。两步策略为在 PCL 移植物表面结合肝素提供了一种简单通用的途径。本工作中展示的表面肝素化 PCL 可为可生物降解的血管支架移植物提供一种有用的材料平台。
