Yang Y R, Yang F, Sun N, Wang G Y
College of Biology and Food Engineering, Huanghuai University, Zhumadian 463000, China.
Department of Basic Veterinary Medicine, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China.
Iran J Vet Res. 2019 Spring;20(2):131-135.
Orbifloxacin is being widely used in China to treat fish infections in an extra-label manner, which may cause its potential residues in edible tissues.
The purpose of this study was to determine the disposition kinetics of orbifloxacin in crucian carp () following intramuscular administration for its further safe application in aquaculture industry.
Tissue samples of skin, muscle, kidney, and liver were collected from six crucian carps reared at 25°C at 5, 10, 15, 30, 45 min, 1, 2, 4, 6, 12, 24, 48, 72, and 96 h following a single intramuscular injection at 7.5 mg/kg body weight (BW). The orbifloxacin concentrations in tissues were determined using a high-performance liquid chromatography (HPLC) method with a fluorescence detector, then average concentrations time data were subjected to non-compartmental analysis to obtain the kinetic parameters.
The peak concentration of 5.68 ± 0.03 µg/g was calculated in kidney at 2 h, followed by muscle (5.51 ± 0.01 µg/g) at 4 h, liver (4.84 ± 0.20 µg/g) at 2 h, and skin (4.27 ± 0.08 µg/g) at 4 h. Area under concentration-time curve was calculated as 79.22, 94.72, 118.65, and 129.02 h·µg/g in kidney, liver, skin, and muscle, respectively. And the elimination half-lives were determined as 18.17, 18.41, 18.77, and 19.11 h in skin, kidney, muscle, and liver, respectively.
It was shown that orbifloxacin was well distributed into tissues while relatively slowly eliminated in crucian carp reared at 25°C following a single intramuscular injection.
在中国，奥比沙星正被广泛用于超说明书范围治疗鱼类感染，这可能导致其在可食用组织中产生潜在残留。
本研究旨在确定奥比沙星在鲫鱼体内肌内注射后的处置动力学，以便其在水产养殖业中进一步安全应用。
在25℃饲养的6条鲫鱼中，以7.5mg/kg体重进行单次肌内注射后，于5、10、15、30、45分钟、1、2、4、6、12、24、48、72和96小时采集皮肤、肌肉、肾脏和肝脏的组织样本。使用带荧光检测器的高效液相色谱（HPLC）法测定组织中奥比沙星的浓度，然后对平均浓度-时间数据进行非房室分析以获得动力学参数。
肾脏在2小时时计算出的峰浓度为5.68±0.03μg/g，其次是肌肉（5.51±0.01μg/g）在4小时时，肝脏（4.84±0.20μg/g）在2小时时，皮肤（4.27±0.08μg/g）在4小时时。肾脏、肝脏、皮肤和肌肉的浓度-时间曲线下面积分别计算为79.22、94.72、118.65和129.02h·μg/g。皮肤、肾脏、肌肉和肝脏的消除半衰期分别确定为18.17、18.41、18.77和19.11小时。
结果表明，在25℃饲养的鲫鱼单次肌内注射后，奥比沙星在组织中分布良好，但消除相对缓慢。
