Entezari Maliheh, Ghanbary Fatemeh
Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
M. E. and F. G. contributed equally to this work.
Iran J Pharm Res. 2019 Spring;18(2):713-719. doi: 10.22037/ijpr.2019.1100639.
The TiO2, which is a main material in the field of photocatalytic reactions, includes rutile and anatase phase. Titanium dioxide has possessed notice due to its promising applications in the environmental photocatalytic degradation of pollutants of organic compound in waste water and utilization of solar energy. The nanosized manganese titanate (pyrophanite) MnTiO3 was collected by oxidation of Mn(OH)2 with TiO2 powder in cetyltrimethylammonium bromide (CTAB) micelle solutions and the calcinations of the produced powders. Therefore, it was decided to determine the Mechanistic mitochondria toxicity of nanoparticles towards liver, kidney, heart, and brain via new and reliable methods. Our results showed that nanoparticles induced mitochondria dysfunction via an increase in ROS production and membrane potential collapse, correlated to cytochrome c release. Also, increased disturbance in oxidative phosphorylation was also shown by the decrease in ATP. Recent studies have suggested that nanoparticles leading to cytosolic release of lysosomal content, and ultimately apoptosis. This study suggests that mitochondrial oxidative stress and impairment of oxidative phosphorylation in vital organ Mitochondria may play a key role in manganese titanate toxicity.
二氧化钛是光催化反应领域的主要材料，包括金红石相和锐钛矿相。二氧化钛因其在废水有机化合物污染物的环境光催化降解以及太阳能利用方面的应用前景而受到关注。通过在十六烷基三甲基溴化铵（CTAB）胶束溶液中用二氧化钛粉末氧化氢氧化锰（Mn(OH)₂）并对所得粉末进行煅烧，收集到了纳米级锰钛矿（焦绿石）MnTiO₃。因此，决定通过新的可靠方法来确定纳米颗粒对肝脏、肾脏、心脏和大脑的线粒体毒性机制。我们的结果表明，纳米颗粒通过增加活性氧（ROS）生成和膜电位崩溃诱导线粒体功能障碍，这与细胞色素c释放相关。此外，ATP减少也表明氧化磷酸化的干扰增加。最近的研究表明，纳米颗粒会导致溶酶体内容物释放到细胞质中，并最终导致细胞凋亡。本研究表明，重要器官线粒体中的线粒体氧化应激和氧化磷酸化损伤可能在锰钛矿毒性中起关键作用。
