Castillo-Silva Brenda Eréndida, Martínez-Jiménez Verónica, Martínez-Castañón Gabriel Alejandro, Medina-Solís Carlo Eduardo, Aguirre-López Eva Concepción, Castillo-Hernández Jesús Ramón, Niño-Martínez Nereyda, Patiño-Marín Nuria
University of San Luis Potosí, Program of Doctorate in Dental Sciences, Department of Clinical Research, San Luis Potosí, SLP, Mexico.
University of San Luis Potosí, Program of Doctorate in Dental Sciences, San Luis Potosí, SLP, Mexico.
Braz Oral Res. 2019 Sep 9;33:e077. doi: 10.1590/1807-3107bor-2019.vol33.0077.
The aim of the present study was to identify the relationship between the expression of calcitonin gene-related peptide (CGRP) and the responses of pulp sensitivity tests in healthy pulps and irreversible pulps by performing a cross-sectional study on patients. Two hundred subjects were evaluated. A total of 75 subjects complied with the criteria. The participants were divided into two groups: a) Healthy pulp (subjects [n = 35] having posterior teeth with clinically normal pulp tissue), and b) Irreversible pulpitis (subjects [n = 40] having posterior teeth with irreversible pulpitis). All participants were evaluated using the following variables: a) medical and dental history, b) pulp sensitivity tests, c) expression of CGRP by the enzyme-linked immunosorbent assay (ELISA), and d) expression levels of mRNA CGRP and mRNA CGRP receptor genes. We determined that the responses of the cold test between 4 and ≥12 s presented a higher average of the expression of CGRP in the group having irreversible pulpitis (p = 0.0001). When we compared the groups with the value of the electrical impulse, we found statistically significant differences (p = 0.0001), observing positive responses to the test with electrical impulses of 7 to 10, with an average of 72.15 ng/mL of CGRP in the irreversible pulpitis group. High values of CGRP expression were observed in that group in the responses of pulp sensitivity.
本研究的目的是通过对患者进行横断面研究，确定健康牙髓和不可逆牙髓中降钙素基因相关肽（CGRP）的表达与牙髓敏感性测试反应之间的关系。对200名受试者进行了评估。共有75名受试者符合标准。参与者被分为两组：a）健康牙髓组（n = 35，后牙牙髓组织临床正常的受试者），和b）不可逆性牙髓炎组（n = 40，患有不可逆性牙髓炎的后牙受试者）。所有参与者均使用以下变量进行评估：a）医学和牙科病史，b）牙髓敏感性测试，c）通过酶联免疫吸附测定（ELISA）检测CGRP的表达，以及d）CGRP mRNA和CGRP受体基因的mRNA表达水平。我们确定，在4至≥12秒之间的冷测试反应中，不可逆性牙髓炎组中CGRP的平均表达较高（p = 0.0001）。当我们比较两组的电冲动值时，发现有统计学显著差异（p = 0.0001），观察到在7至10的电冲动测试中有阳性反应，不可逆性牙髓炎组中CGRP的平均含量为72.15 ng/mL。在该组牙髓敏感性反应中观察到CGRP的高表达值。
