gen. nov., sp. nov., a new member of the family from a karstic cavern.

A novel actinobacterium, designated strain CFH 30434, was isolated from a soil sample collected from a karst cave in Luoyang, Henan Province, PR China. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of the strain were aerobic, Gram-stain-positive, non-motile and coccoid or short rods. The strain was found to be oxidase-positive and weakly catalase-positive. Strain CFH 30434 grew optimally at 28 °C, pH 7.0-9.0 and in the presence of up to 0-1.5 % NaCl (w/v). The whole-cell sugars were glucose, mannose and rhamnose. The major isoprenoid quinone was MK-9(H) and the major fatty acids (>10 % of the total fatty acids compositions) were anteiso-C, iso-C and iso-C. The polar lipids detected were diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The genomic DNA G+C content was determined to be 72.3 mol%. The results of phylogenetic analysis of 16S rRNA gene sequences indicated that CFH 30434 clustered within the family , and closely with the type strains of DSM 12657, DSM 17134 and CCTCC AA 208046 (97.3 %, 96.2 and 95.9 % sequence similarities, respectively). Phylogenetic analysis showed that strain CFH 30434 formed a separate evolutionary branch, and was parallel to other related genera of . Its phylogenetic distinctiveness and distinguishing phenotypic characteristics supported that strain CFH 30434 represents a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CFH 30434 (=CGMCC 1.13856=KCTC 49093).