Key Laboratory of Pu-Er Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China.
College of Food Science and Technology, Yunnan Agricultural University, Kunming, 650201, China.
Arch Microbiol. 2021 May;203(4):1509-1518. doi: 10.1007/s00203-020-02151-z. Epub 2021 Jan 4.
A Gram-staining positive aerobic bacterium, designated TLY-12, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China. Strain TLY-12 grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%). The major cellular fatty acids were anteiso-C, C and iso-C. The respiratory quinone were menaquinones MK-9 (H) and MK-9 (H). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI), phosphoglycolipid (PGL), glycolipid (GL) and an unidentified phospholipid (PL). The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid. Whole-cell sugars of the isolate were ribose, galactose and glucose. Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603, which gave sequence similarity of 97.9%. Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%. Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species. Genome phylogenetic analysis showed that strain TLY-12 formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae. Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12 is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp. nov. is proposed. The type strain is TLY-12 (= CGMCC 1.17157 = KCTC 49467).
一株革兰氏阳性需氧细菌，被命名为 TLY-12，从中国云南省普洱市普洱茶堆发酵过程中分离得到。菌株 TLY-12 在 15-37°C（最佳温度 30°C）、pH6.0-11.0（最佳 pH9.0）和 0-9.0%（w/v）NaCl（最佳 3.0%）的条件下生长。主要的细胞脂肪酸为 anteiso-C、C 和 iso-C。呼吸醌为menaquinones MK-9（H）和 MK-9（H）。极性脂类为磷脂酰甘油（PG）、双磷脂酰甘油（DPG）、磷脂酰肌醇（PI）、磷糖脂（PGL）、糖脂（GL）和一种未鉴定的磷脂（PL）。肽聚糖含有谷氨酸、天冬氨酸、丙氨酸和赖氨酸，其中赖氨酸是诊断性二氨基酸。该菌株的全细胞糖为核糖、半乳糖和葡萄糖。16S rRNA 基因的系统发育分析表明，该菌株属于 Promicromonosporaceae 科，与 Isoptericola cucumis DSM 101603 最为密切相关，其序列相似性为 97.9%。基因组测序显示基因组大小为 3.91 Mbp，G+C 含量为 75.0%。平均核苷酸同一性和数字 DNA-DNA 杂交值均低于描述的 Promicromonosporaceae 种的种阈值。基因组系统发育分析表明，菌株 TLY-12 形成了一个独立的进化分支，与 Promicromonosporaceae 科的其他相关属平行。基于系统发育、表型、化学分类和基因组成对数据，菌株 TLY-12 被认为代表了 Promicromonosporaceae 科中一个新属的新种，命名为 Puerhibacterium puerhi gen. nov.，sp. nov.。模式菌株为 TLY-12（=CGMCC 1.17157=KCTC 49467）。
