National Engineering Research Center of Marine Facilities Aquaculture, College of Marine Science, Zhejiang Ocean University, No. 1 Haida South Road, Dinghai District, Zhoushan, Zhejiang Province, 316022, China.
Administration of Ocean and Fisheries of Zhoushan, No 21,Chenghe xi Road, Dinghai District, Zhoushan, Zhejiang Province, 316021, China; School of Marine Sciences Ningbo University, No 818 Fenghua Road, Jiangbai District, Ningbo City, Zhejiang Province, 315211, China.
Fish Shellfish Immunol. 2019 Nov;94:634-642. doi: 10.1016/j.fsi.2019.09.015. Epub 2019 Sep 15.
Long non-coding RNA refers to an RNA transcript of a non-coding protein with a sequence length greater than 200 bp. More and more reports indicated that lncRNA was involved in the regulation of gene expression as a signalling molecule, an inducing molecule, a leader molecule and a scaffold molecule. Previous studies have sequenced the draft genome and several transcriptome data sets for protein-coding genes of the large yellow croaker (Larimichthys crocea), but little is known about the expression and function of lncRNAs in this species. In order to obtain a catalogue of lncRNAs for this croaker, Vibrio parahaemolyticus infection challenge experiment was conducted and long non-coding RNA sequences were obtained. Using high-throughput sequencing of lncRNA, a total of 73,233 high-confidence transcripts were reconstructed in 32,726 loci, recovering most of the expressed reference transcripts, and 6473 novel expressed loci were identified. The tissue expression profile revealed that most lacunas were specifically enriched in distinct tissues. A set of 163 lncRNAs were identified as being specifically expressed in the spleen and may be involved in the immune response. It is the first time to identify specific lncRNAs in the L. crocea systematically in this croaker, aiming to benefit the future genomic study of this species.
长链非编码 RNA 是指序列长度大于 200bp 的不编码蛋白质的 RNA 转录本。越来越多的报道表明，lncRNA 作为信号分子、诱导分子、引导分子和支架分子参与基因表达的调控。先前的研究已经对大黄鱼（Larimichthys crocea）的蛋白质编码基因进行了基因组草图和几个转录组数据集的测序，但对该物种中 lncRNA 的表达和功能知之甚少。为了获得大黄鱼的 lncRNA 目录，进行了副溶血弧菌感染挑战实验，并获得了长链非编码 RNA 序列。通过高通量 lncRNA 测序，在 32726 个基因座中重建了 73233 个高可信度转录本，恢复了大多数表达的参考转录本，并鉴定了 6473 个新的表达基因座。组织表达谱显示，大多数 lacunas 特异性富集在不同的组织中。一组 163 个 lncRNAs 被鉴定为在脾脏中特异性表达，可能参与免疫反应。这是首次在大黄鱼中系统地鉴定特定的 lncRNAs，旨在为该物种的未来基因组研究提供帮助。
