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提取物可保护过氧化氢诱导的人视网膜色素上皮细胞死亡及膜通透性。

Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability.

作者信息

Kang Nu Ri, Pyun Bo-Jeong, Jung Dong Ho, Lee Ik Soo, Kim Chan-Sik, Kim Young Sook, Kim Jin Sook

机构信息

Herbal Medicine Division, Korea Institute of Oriental Medicine (KIOM), Daejeon, Republic of Korea.

Korean Medicine Life Science, University of Science Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea.

出版信息

Evid Based Complement Alternat Med. 2019 Aug 27;2019:5710289. doi: 10.1155/2019/5710289. eCollection 2019.

DOI:10.1155/2019/5710289
PMID:31534464
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6732599/
Abstract

BACKGROUND

Pueraria is used in traditional Asian medicine to treat cardiovascular diseases, diarrhea, diabetes mellitus, and diabetic complications such as diabetic retinopathy. Oxidative stress in retinal pigment epithelial cells is implicated in the pathogenesis of retinopathy and age-related macular degeneration (AMD). Here, we evaluated whether the extract can prevent cell death and decrease membrane permeability in oxidative stress-induced human retinal pigment epithelial cells.

METHODS

The effects of extract on hydrogen peroxide- (HO-) induced oxidative stress were investigated using 2',7'-dichlorofluorescin diacetate, western blotting, and immunohistochemistry in human retinal pigment epithelial cells. The effects of puerarin, daidzein, and daidzin isolated from extract were also studied by determining cell death, reactive oxygen species (ROS) generation, and p38 mitogen-activated protein kinase (MAPK) and c-Jun -terminal kinase (JNK) phosphorylation.

RESULTS

Our results showed that the extract inhibited ROS generation, suppressed the disruption of zonula occludens-1 (ZO-1), and reduced membrane permeability in HO-induced human retinal pigment epithelial cells. Additionally, the extract prevented the inhibition of p38 MAPK and JNK phosphorylation.

CONCLUSION

Our findings suggest that the extract has the potential to prevent AMD development by inhibiting the mechanism underlying oxidative stress-mediated ocular disorders.

摘要

背景

葛根在亚洲传统医学中用于治疗心血管疾病、腹泻、糖尿病以及糖尿病并发症，如糖尿病视网膜病变。视网膜色素上皮细胞中的氧化应激与视网膜病变和年龄相关性黄斑变性（AMD）的发病机制有关。在此，我们评估了[提取物名称]提取物是否能预防氧化应激诱导的人视网膜色素上皮细胞死亡并降低膜通透性。

方法

在人视网膜色素上皮细胞中，使用2',7'-二氯荧光素二乙酸酯、蛋白质印迹法和免疫组织化学法研究了[提取物名称]提取物对过氧化氢（H₂O₂）诱导的氧化应激的影响。还通过测定细胞死亡、活性氧（ROS）生成以及p38丝裂原活化蛋白激酶（MAPK）和c-Jun氨基末端激酶（JNK）磷酸化，研究了从[提取物名称]提取物中分离出的葛根素、大豆苷元和大豆苷的作用。

结果

我们的结果表明，[提取物名称]提取物抑制了ROS生成，抑制了紧密连接蛋白1（ZO-1）的破坏，并降低了H₂O₂诱导的人视网膜色素上皮细胞的膜通透性。此外，[提取物名称]提取物阻止了p38 MAPK和JNK磷酸化的抑制。

结论

我们的研究结果表明，[提取物名称]提取物有可能通过抑制氧化应激介导的眼部疾病的潜在机制来预防AMD的发展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/69a5ed6840f9/ECAM2019-5710289.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/9b6cfba887ca/ECAM2019-5710289.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/d75eb15a1c60/ECAM2019-5710289.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/a0973f9a79fe/ECAM2019-5710289.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/9814c4720939/ECAM2019-5710289.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/1b59d8aac17f/ECAM2019-5710289.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/69a5ed6840f9/ECAM2019-5710289.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/9b6cfba887ca/ECAM2019-5710289.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/d75eb15a1c60/ECAM2019-5710289.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/a0973f9a79fe/ECAM2019-5710289.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/9814c4720939/ECAM2019-5710289.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/1b59d8aac17f/ECAM2019-5710289.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c9c/6732599/69a5ed6840f9/ECAM2019-5710289.006.jpg

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2
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J Tissue Eng. 2016 Jul 12;7:2041731416650838. doi: 10.1177/2041731416650838. eCollection 2016 Jan-Dec.
3
Artemisinin protects human retinal pigment epithelial cells from hydrogen peroxide-induced oxidative damage through activation of ERK/CREB signaling.
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Anim Nutr. 2022 Jul 14;11:48-59. doi: 10.1016/j.aninu.2022.06.014. eCollection 2022 Dec.
4
Discovering the Potential of Natural Antioxidants in Age-Related Macular Degeneration: A Review.探索天然抗氧化剂在年龄相关性黄斑变性中的潜力：综述
Pharmaceuticals (Basel). 2022 Jan 14;15(1):101. doi: 10.3390/ph15010101.
青蒿素通过激活ERK/CREB信号通路保护人视网膜色素上皮细胞免受过氧化氢诱导的氧化损伤。
Redox Biol. 2016 Oct;9:50-56. doi: 10.1016/j.redox.2016.06.002. Epub 2016 Jun 20.
4
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6
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7
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8
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Phytother Res. 2014 Jul;28(7):961-75. doi: 10.1002/ptr.5083. Epub 2013 Dec 13.
9
Induction of necrotic cell death by oxidative stress in retinal pigment epithelial cells.氧化应激诱导视网膜色素上皮细胞坏死性细胞死亡。
Cell Death Dis. 2013 Dec 12;4(12):e965. doi: 10.1038/cddis.2013.478.
10
The blood-retina barrier: tight junctions and barrier modulation.血视网膜屏障：紧密连接和屏障调节。
Adv Exp Med Biol. 2012;763:70-84.