Long Yu, He Yuqing, Jie Fengming, Li Sixin, Wu Yanli, Li Yanwu, Du Qun
Pi-Wei Institute, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
Evid Based Complement Alternat Med. 2019 Aug 25;2019:7837989. doi: 10.1155/2019/7837989. eCollection 2019.
To investigate the effect of Kuijieling (KJL) on the balance between T helper 17 (Th17) and regulatory T (Treg) cells in peripheral blood mononuclear cells (PBMC) and explore the underlying mechanism.
PBMCs isolated from rats were stimulated with transforming growth factor-, interleukin (IL)-6, and IL-23 to induce the imbalance of Th17 and Treg cells and were treated with 10, 5, or 2.5% KJL-containing serum. The proportion of Th17 or Treg cells in CD4 T cells was analyzed by flow cytometry, the concentrations of IL-17, IL-21, and IL-10 were assayed by ELISA, mRNA expressions of retinoic acid-related orphan receptor t (RORt), forkhead box protein 3 (Foxp3), and signal transducer and activator of transcription 3 (STAT3) were quantified by PCR, and phosphorylated STAT3 (p-STAT3) was analyzed by flow cytometry.
KJL-containing serum decreased the proportion of Th17 cells and increased the proportion of Treg cells in CD4 T cells, decreased the concentration of IL-17 and IL-21, enhanced the level of IL-10 in the cell culture supernatant, promoted the expression of Foxp3, and inhibited the levels of RORt, STAT3, and p-STAT3.
KJL suppresses the STAT3 pathway to remedy the imbalance between Th17 and Treg cells.
探讨溃结灵（KJL）对外周血单个核细胞（PBMC）中辅助性T细胞17（Th17）与调节性T（Treg）细胞平衡的影响，并探究其潜在机制。
用转化生长因子、白细胞介素（IL）-6和IL-23刺激从大鼠分离的PBMC，以诱导Th17和Treg细胞失衡，并用含10%、5%或2.5%KJL的血清处理。通过流式细胞术分析CD4 T细胞中Th17或Treg细胞的比例，用酶联免疫吸附测定法检测IL-17、IL-21和IL-10的浓度，用聚合酶链反应定量视黄酸相关孤儿受体t（RORt）、叉头框蛋白3（Foxp3）和信号转导子与转录激活子3（STAT3）的mRNA表达，并用流式细胞术分析磷酸化STAT3（p-STAT3）。
含KJL的血清降低了CD4 T细胞中Th17细胞的比例，增加了Treg细胞的比例，降低了IL-17和IL-21的浓度，提高了细胞培养上清液中IL-10的水平，促进了Foxp3的表达，并抑制了RORt、STAT3和p-STAT3的水平。
KJL通过抑制STAT3信号通路纠正Th17和Treg细胞之间的失衡。
