Maihle N J, Satir B H
Albert Einstein College of Medicine, Department of Anatomy and Structural Biology, Bronx, New York 10461.
J Cell Biochem. 1986;31(3):195-202. doi: 10.1002/jcb.240310302.
Tetrahymena thermophila is a ciliated protozoan studied by investigators from a wide range of disciplines as a model system for a variety of specialized eukaryotic cell functions. The proteinaceous secretory products of T thermophila have been isolated and characterized and in this study we identify the major secretory product, a 34,000 Mr polypeptide, and use an antiserum prepared against this secretory protein to (1) demonstrate that this 34,000 Mr polypeptide is truly a secreted protein in Tetrahymena and (2) monitor the synthesis and transport of this protein by indirect immunofluorescence and light microscopy during mucocyst biogenesis.
嗜热四膜虫是一种纤毛原生动物，被来自广泛学科的研究人员作为各种特殊真核细胞功能的模型系统进行研究。嗜热四膜虫的蛋白质分泌产物已被分离和鉴定，在本研究中，我们鉴定了主要分泌产物，一种分子量为34000的多肽，并使用针对这种分泌蛋白制备的抗血清来：（1）证明这种分子量为34000的多肽在嗜热四膜虫中确实是一种分泌蛋白；（2）在黏液囊泡生物发生过程中，通过间接免疫荧光和光学显微镜监测该蛋白的合成和运输。
