Department of Electronic Engineering, Gachon University, Seongnam-si, Gyeonggi-do, 13120, South Korea.
Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, 21999, Republic of Korea.
Biosens Bioelectron. 2019 Nov 1;144:111694. doi: 10.1016/j.bios.2019.111694. Epub 2019 Sep 7.
In this study, we developed a small size, low cost, highly sensitive electrochemical biosensor with a low limit of detection by immobilizing specific anti-amyloid-β (aβ) antibody onto a self-assembled monolayer functionalized interdigitated chain-shaped electrode (anti-aβ/EDC-NHS/SAM/ICE). The anti-aβ/EDC-NHS/SAM/ICE specifically detects aβ 1-42 peptide (a peptide 1-42 amino acids long), which is one of main biomarkers of Alzheimer's disease in human serum (HS). Electrochemical impedance spectroscopy (EIS) was used to characterize the impedance change of the anti-aβ/EDC-NHS/SAM/ICE biosensor for aβ 1-42 detection, which provided a wide linear range of detection from 10-10 ng mL, and a low limit of detection of aβ in HS (100 pg mL) much lower than the limit of detection of CSF aβ 1-42 (∼500 pg mL), and other biosensors. Therefore, the developed biosensor is sensitive enough to be used for the diagnosis of early stage Alzheimer's disease.
在这项研究中，我们通过将特异性抗淀粉样蛋白-β（aβ）抗体固定在自组装单分子功能化的叉指链状电极（抗 aβ/EDC-NHS/SAM/ICE）上，开发了一种小型、低成本、高灵敏度的电化学生物传感器，其检测限较低。抗 aβ/EDC-NHS/SAM/ICE 特异性检测人血清（HS）中阿尔茨海默病的主要生物标志物之一的 aβ 1-42 肽（一种 1-42 个氨基酸长的肽）。电化学阻抗谱（EIS）用于表征抗 aβ/EDC-NHS/SAM/ICE 生物传感器用于检测 aβ 1-42 的阻抗变化，该传感器提供了从 10-10 ng/mL 宽线性检测范围，HS 中 aβ 的检测限较低（100 pg/mL），低于脑脊液 aβ 1-42 的检测限（约 500 pg/mL）和其他生物传感器。因此，开发的生物传感器足够灵敏，可用于早期阿尔茨海默病的诊断。
