Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, 27709, USA.
Sci Rep. 2019 Sep 20;9(1):13618. doi: 10.1038/s41598-019-50052-z.
Spermatogenesis is a differentiation process that requires dramatic changes to DNA architecture, a process governed in part by Transition Nuclear Proteins 1 and 2 (TNP1 and TNP2). Translation of Tnp1 and Tnp2 mRNAs is temporally disengaged from their transcription. We hypothesized that RNA regulatory proteins associate specifically with Tnp mRNAs to control the delayed timing of their translation. To identify potential regulatory proteins, we isolated endogenous mRNA/protein complexes from testis extract and identified by mass spectrometry proteins that associated with one or both Tnp transcripts. Five proteins showed strong association with Tnp transcripts but had low signal when Actin mRNA was isolated. We visualized the expression patterns in testis sections of the five proteins and found that each of the proteins was detected in germ cells at the appropriate stages to regulate Tnp RNA expression.
精子发生是一个需要 DNA 结构发生巨大变化的分化过程，这一过程部分受过渡核蛋白 1 和 2（TNP1 和 TNP2）调控。TNP1 和 TNP2mRNA 的翻译与转录在时间上是脱钩的。我们假设 RNA 调节蛋白特异性地与 TnpmRNA 结合，以控制其翻译的延迟时间。为了鉴定潜在的调节蛋白，我们从睾丸提取物中分离内源性 mRNA/蛋白复合物，并通过质谱法鉴定与一个或两个 Tnp 转录本结合的蛋白。有 5 种蛋白与 Tnp 转录本强烈结合，但当分离肌动蛋白 mRNA 时，其信号较弱。我们在睾丸组织切片中观察了这 5 种蛋白的表达模式，发现每种蛋白都在适当的阶段在生殖细胞中被检测到，以调节 TnpRNA 的表达。
