Department of Biological Engineering, Inha University, Incheon, 22212, Republic of Korea.
Biotechnol Lett. 2019 Nov;41(11):1255-1263. doi: 10.1007/s10529-019-02728-9. Epub 2019 Sep 20.
To examine the effects of suramin in CHO cell cultures in terms of the cell culture performance and quality of the Fc-fusion protein.
Suramin had positive effects on the CHO cell cultures. The addition of suramin caused an increase in the viable cell density, cell viability, and titer of the Fc-fusion protein. Moreover, suramin had no impact on protein aggregation and enhanced the sialic acid contents of Fc-fusion protein by 1.18-fold. The enhanced sialylation was not caused by the increased nucleotide sugar level but by the inhibition of sialidase activity. The results showed that suramin inhibited apoptosis and had positive impacts on the productivity and quality of Fc-fusion protein.
The addition of suramin increased the production of Fc-fusion protein and enhanced sialylation when added as a supplement to the media component in CHO cell cultures. This study suggested that suramin could be a beneficial additive during the biological production in terms of the productivity and quality of Fc-fusion protein.
研究苏拉明对 CHO 细胞培养的影响，考察其对细胞培养性能和 Fc 融合蛋白质量的影响。
苏拉明对 CHO 细胞培养具有积极作用。添加苏拉明可提高活细胞密度、细胞活力和 Fc 融合蛋白效价。此外，苏拉明对蛋白聚集无影响，可使 Fc 融合蛋白的唾液酸含量提高 1.18 倍。这种增强的唾液酸化不是由于核苷酸糖水平的增加，而是由于唾液酸酶活性的抑制。结果表明，苏拉明抑制细胞凋亡，对 Fc 融合蛋白的产率和质量具有积极影响。
苏拉明作为培养基成分的添加物可提高 CHO 细胞培养中 Fc 融合蛋白的产量，并增强其唾液酸化程度。本研究表明，苏拉明在生物生产中可作为一种有益的添加剂，提高 Fc 融合蛋白的产率和质量。
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