用于中国仓鼠卵巢细胞中重组蛋白表达的无血清培养基。
Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells.
作者信息
Li Weifeng, Fan Zhenlin, Lin Yan, Wang Tian-Yun
机构信息
Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, China.
International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan, Xinxiang Medical University, Xinxiang, China.
出版信息
Front Bioeng Biotechnol. 2021 Mar 15;9:646363. doi: 10.3389/fbioe.2021.646363. eCollection 2021.
At present, nearly 70% of recombinant therapeutic proteins (RTPs) are produced by Chinese hamster ovary (CHO) cells, and serum-free medium (SFM) is necessary for their culture to produce RTPs. In this review, the history and key components of SFM are first summarized, and its preparation and experimental design are described. Some small molecule compound additives can improve the yield and quality of RTP. The function and possible mechanisms of these additives are also reviewed here. Finally, the future perspectives of SFM use with CHO cells for RTP production are discussed.
目前，近70%的重组治疗性蛋白（RTPs）是由中国仓鼠卵巢（CHO）细胞生产的，无血清培养基（SFM）是培养这些细胞以生产RTPs所必需的。在这篇综述中，首先总结了SFM的历史和关键成分，并描述了其制备方法和实验设计。一些小分子化合物添加剂可以提高RTP的产量和质量。本文还综述了这些添加剂的功能和可能的作用机制。最后，讨论了SFM用于CHO细胞生产RTP的未来前景。
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