Zhang Xinyan, Zhao Meixia, Lisch Damon
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, USA.
Center for Plant Biology, Purdue University, West Lafayette, IN, USA.
Methods Mol Biol. 2020;2072:39-50. doi: 10.1007/978-1-4939-9865-4_5.
Transposable elements can be highly mutagenic because when they transpose they can insert into genes and disrupt their function, a propensity which has been exploited in many organisms to generate tagged mutant alleles. The Mutator (Mu) family transposon is a family of DNA-type transposons in maize with a particularly high duplication frequency, which results in large numbers of new mutations in lineages that carry active Mu elements. Here we describe a rapid and cost-effective Miseq-based Mu transposon profiling pipeline. This method can also be used for identifying flanking sequences of other types of long insertions such as T-DNAs.
转座元件可能具有高度致突变性，因为当它们转座时，能够插入基因并破坏其功能，许多生物体利用这种特性来产生带标签的突变等位基因。Mutator（Mu）家族转座子是玉米中的一类DNA型转座子，其复制频率特别高，这导致携带活性Mu元件的谱系中出现大量新突变。在这里，我们描述了一种基于Miseq的快速且经济高效的Mu转座子分析流程。该方法还可用于鉴定其他类型长插入片段（如T-DNA）的侧翼序列。
