Soto-Cardinault Cynthia G, Duarte-Aké Fátima, De-la-Peña Clelia, Góngora-Castillo Elsa
Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.
CONACYT-Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.
Methods Mol Biol. 2020;2072:65-84. doi: 10.1007/978-1-4939-9865-4_7.
RNA sequencing (RNA-seq) coupled to DNA methylation strategies enables the detection and characterization of genes which expression levels might be mediated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or developmental stage.
将RNA测序（RNA-seq）与DNA甲基化策略相结合，能够检测和鉴定那些表达水平可能受DNA甲基化介导的基因。在此，我们描述了一种生物信息学方法，用于利用RNA-seq数据分析基因表达水平，从而使我们能够识别出可通过亚硫酸氢盐分析进行检测的候选基因。候选甲基化基因通常是那些在特定条件或发育阶段低表达的基因。
