Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland; Laboratory of Applied Periodontal and Peri-implantitis Sciences, Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Arch Oral Biol. 2020 Jan;109:104552. doi: 10.1016/j.archoralbio.2019.104552. Epub 2019 Sep 12.
The aim of this in vitro study was to investigate the behavior of osteoblasts on titanium discs under different concentrations of enamel matrix derivatives (EMD) and dentin matrix derivative (DMD).
MC3T3-E1 osteoblast-like cells were cultivated on coated titanium SLA discs with EMD or DMD at 100 μg/ml, 1 mg/ml, 10 mg/ml and 30 mg/ml or left uncoated. Cell viability, proliferation, adhesion and migration were assessed respectively with MTT, BrdU, DAPI and scratch wound healing assays. Messenger ribonucleic acid of different genes related to osteoblastic differentiation was quantified by means of real-time quantitative PCR. Data were analyzed using student t-test for adhesion and migration assay and ANOVA for proliferation assay (p < 0.05).
BrdU incorporation was found in proliferative osteoblasts for both test solutions at all concentrations. Osteoblast migrated and covered approximately 70% of the wound area observed at time zero when exposed to EMD and DMD to all concentrations. The increase of gene expression was dependent on the concentration enhancement of EMD and DMD. Higher concentrations showed proliferation augmentation if compared to lower concentrations.
Roughness surface of Ti SLA can limit cell adhesion independent of the presence EMD or DMD. DMD enhances cell migration of osteoblasts on SLA titanium implants in a concentration-dependent manner.
本体外研究旨在探讨不同浓度釉基质衍生物（EMD）和牙本质基质衍生物（DMD）对钛盘上成骨细胞行为的影响。
将 MC3T3-E1 成骨样细胞培养在涂有 EMD 或 DMD 的 SLA 钛盘上，浓度分别为 100μg/ml、1mg/ml、10mg/ml 和 30mg/ml，或不涂覆。分别采用 MTT、BrdU、DAPI 和划痕愈合试验评估细胞活力、增殖、黏附和迁移。采用实时定量 PCR 定量检测不同骨分化相关基因的信使核糖核酸。采用学生 t 检验分析黏附和迁移试验的数据，采用 ANOVA 分析增殖试验的数据（p<0.05）。
两种测试溶液在所有浓度下均能使增殖性成骨细胞掺入 BrdU。当暴露于 EMD 和 DMD 时，成骨细胞迁移并覆盖了观察到的零时伤口面积的约 70%。基因表达的增加取决于 EMD 和 DMD 浓度的增强。与低浓度相比，高浓度显示出增殖的增加。
SLA 钛表面的粗糙度可以限制细胞黏附，而与 EMD 或 DMD 的存在无关。DMD 以浓度依赖的方式增强 SLA 钛种植体上成骨细胞的迁移。
