Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province, China.
Department of Anesthesiology, Kunming Angel Women's & Children's Hospital, Kunming, Yunnan Province, China.
Biomed Pharmacother. 2019 Dec;120:109463. doi: 10.1016/j.biopha.2019.109463. Epub 2019 Sep 18.
Dexmedetomidine is widely used for perioperative and ICU patients. microRNAs (miRNAs) function as regulators of gene expression. The aim of the study was to assay expression profiling of microRNA in rat hearts following administration of dexmedetomidine.
In this study 6 rats were randomly divided into two groups (n = 3): dexmedetomidine group and control group. The rats of dexmedetomidine group were intraperitoneally given dexmedetomidine in a dose of 100 μg/kg whereas the rats in control group were administered normal saline intraperitoneally. The hearts were excised 30 min after the administration of dexmedetomidine or normal saline under anesthesia. The samples were analyzed for differentially expressed microRNAs with Exiqon miRNA Array. The differentially expressed microRNAs were confirmed by using qRT-PCR. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to find the target genes and signaling pathways of the aberrantly expressed miRNAs.
Six microRNAs were identified to be significantly expressed, among of which, five microRNAs (miRNA-434-3p, miRNA-3596d, miRNA-496-5p, miRNA-7a-2-3p and miRNA-702-3p) were up-regulated and 1 microRNA (miRNA-208b-3p) down-regulated compared to those of control group. The aberrantly expressed microRNAs were further validated by Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). GO and KEGG analyses were used to identify target genes and the signaling pathways.
The use of dexmedetomidine is associated with differentially expressed microRNAs which may be involved in cardioprotection following administration of dexmedetomidine.
右美托咪定广泛用于围手术期和 ICU 患者。微小 RNA（miRNA）作为基因表达的调节剂。本研究的目的是检测右美托咪定给药后大鼠心脏中 microRNA 的表达谱。
在这项研究中，将 6 只大鼠随机分为两组（n=3）：右美托咪定组和对照组。右美托咪定组大鼠腹腔内给予右美托咪定 100μg/kg，对照组大鼠腹腔内给予生理盐水。麻醉下在给予右美托咪定或生理盐水 30 分钟后取出心脏。使用 Exiqon miRNA 阵列分析差异表达的 microRNA。通过 qRT-PCR 验证差异表达的 microRNA。进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析，以找到异常表达的 microRNA 的靶基因和信号通路。
鉴定出 6 个 microRNA 显著表达，其中 5 个 microRNA（miRNA-434-3p、miRNA-3596d、miRNA-496-5p、miRNA-7a-2-3p 和 miRNA-702-3p）上调，1 个 microRNA（miRNA-208b-3p）下调与对照组相比。通过定量实时聚合酶链反应（qRT-PCR）进一步验证了异常表达的 microRNA。GO 和 KEGG 分析用于鉴定靶基因和信号通路。
右美托咪定的使用与差异表达的 microRNA 相关，这些 microRNA 可能参与右美托咪定给药后的心脏保护作用。
