College of Life Science, Hebei University, Baoding 071002, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
J Chromatogr A. 2020 Jan 11;1610:460540. doi: 10.1016/j.chroma.2019.460540. Epub 2019 Sep 14.
Silver magnetic amino silicone adhesive (FeO@SiO@NH@Ag) particles were prepared for the purification of α-linolenic acid from tree peony seed oil under applied magnetic field. First, FeO@SiO@NH@Ag particles were prepared and physicochemically characterized, including XRD, TG, FTIR, SEM, magnetic hysteresis curves and elemental analysis. The static process for the purification of α-linolenic acid using FeO@SiO@NH@Ag particles was investigated, including adsorption curve, desorption curve, elution solvent composition and adsorption isotherm. The result indicated that 0-1-4% acetone-n-hexane elution solvent was selected for the gradient elution process, 2 h and 60 min were the time required to reach adsorption and desorption equilibrium, 20 °C was selected as the adsorption temperature, Langmuir model was suitable to fit and explain the equilibrium data, and the adsorption process was spontaneous and exothermic. Under applied magnetic field, the dynamic process for the purification of α-linolenic acid using FeO@SiO@NH@Ag particles was investigated, and the optimum conditions were 20:1 µL/g loading amount, 0.5 mL/min flow rate and 51.73 Oe magnetic field intensity. After purification, the purity and recovery ratio of α-linolenic acid were calculated to be 94% and 74%, respectively. Furthermore, the recycled FeO@SiO@NH@Ag particles still achieved better purification result. Therefore, the developed method shows a good application prospect in the field of separation and purification of α-linolenic acid.
银磁性氨基硅酮胶粘剂（FeO@SiO@NH@Ag）颗粒在磁场应用下，用于从牡丹籽油中纯化α-亚麻酸。首先，制备并物理化学表征了 FeO@SiO@NH@Ag 颗粒，包括 XRD、TG、FTIR、SEM、磁滞回线和元素分析。研究了 FeO@SiO@NH@Ag 颗粒用于纯化α-亚麻酸的静态过程，包括吸附曲线、解吸曲线、洗脱溶剂组成和吸附等温线。结果表明，选择 0-1-4%丙酮-正己烷洗脱溶剂进行梯度洗脱过程，达到吸附和解吸平衡所需的时间分别为 2 h 和 60 min，选择 20°C 作为吸附温度，Langmuir 模型适合拟合和解释平衡数据，吸附过程是自发和放热的。在磁场应用下，研究了 FeO@SiO@NH@Ag 颗粒用于纯化α-亚麻酸的动态过程，最佳条件为 20:1 µL/g 的加载量、0.5 mL/min 的流速和 51.73 Oe 的磁场强度。纯化后，计算α-亚麻酸的纯度和回收率分别为 94%和 74%。此外，回收的 FeO@SiO@NH@Ag 颗粒仍能达到较好的纯化效果。因此，所开发的方法在α-亚麻酸分离纯化领域具有良好的应用前景。
