Beller D I, Unanue E R
J Immunol. 1979 Dec;123(6):2890-3.
Optimal receptivity of thymocytes to the mitogenic stimulus in macrophage culture fluid (MCF) is limited to the time in culture when these cells are spontaneously proliferating. By providing a brief, nonmitogenic lectin pulse, nonproliferating cells can be "activated" and their ability to respond to MCF totally restored. This procedure provides a model system for evaluating thymocyte proliferation and, in particular, the mechanism of action of MCF.
胸腺细胞对巨噬细胞培养液（MCF）促有丝分裂刺激的最佳反应性仅限于培养过程中这些细胞自发增殖的时间段。通过提供短暂的、无促有丝分裂作用的凝集素脉冲，可以“激活”非增殖细胞，并使其对MCF的反应能力完全恢复。该程序提供了一个用于评估胸腺细胞增殖，特别是MCF作用机制的模型系统。
