Priya B N V, Venkata Reddy T, Chidanand U, Saiprasad G V S
ITC Limited, ITC Life Sciences & Technology Centre, Peenya Industrial Area, Bengaluru 560 058, India.
J Genet. 2019 Sep;98.
Cytoplasmic male sterility (CMS) is an important trait for large-scale hybrid seed production which avoids manual emasculation and undesired horizontal spread of pollen. Rearrangements in mitochondrial genome in terms of deletions and insertions are frequent causes leading to CMS. Mitochondrial ATP synthase is a multisubunit molecular machine which is involved in synthesis of ATP. In this study, three mutations in ATPase subunit 6 were identified and their cosegregation with male sterility was established using tobacco male sterile hybrids and . A breeder friendly Kompetitive allele specific polymerase chain reaction (KASP) SNP marker was developed for high throughput and quick genotyping. Introgression of this trait into selected germplasm lines ( = 9) was achieved based on foreground for CMS and background selection for recurrent parent using KASP marker and 50K custom tobacco SNP array, respectively. Analysis of genotyping data from SNP array revealed the presence of 88-99% of recurrent parent genome in BCF plants. The selected BCF plants exhibit CMS and are indistinguishable from the fertile recurrent parent (germplasm) in terms of plant morphology.
细胞质雄性不育（CMS）是大规模杂交种子生产的一个重要性状，它避免了人工去雄以及花粉不必要的横向传播。线粒体基因组在缺失和插入方面的重排是导致CMS的常见原因。线粒体ATP合酶是一种参与ATP合成的多亚基分子机器。在本研究中，利用烟草雄性不育杂种鉴定出了ATP酶亚基6中的三个突变，并确定了它们与雄性不育的共分离关系。开发了一种对育种者友好的竞争性等位基因特异性聚合酶链反应（KASP）SNP标记，用于高通量和快速基因分型。分别基于CMS的前景选择和使用KASP标记及50K定制烟草SNP阵列对轮回亲本的背景选择，将该性状导入选定的种质系（n = 9）。对SNP阵列基因分型数据的分析表明，在回交一代（BCF）植株中存在88 - 99%的轮回亲本基因组。所选的BCF植株表现出CMS，并且在植株形态方面与可育的轮回亲本（种质）没有区别。
