Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Capital Medical University, Beijing, China.
Hunan University of Chinese Medicine, Changsha, Hunan Province, China.
Biomed Pharmacother. 2019 Oct;118:109253. doi: 10.1016/j.biopha.2019.109253. Epub 2019 Aug 27.
To investigate the regulation mechanism of baicalin on triple negative breast cancer (TNBC)'s biological network by a systematic biological strategy and cytology experiment.
A systematic biological methodology is utilized to predict the potential targets of baicalin, collect the genes of TNBC, and analyze the TNBC and baicalin's network. After the systematic biological analysis is performed, the cytology experiment, real-time quantitative PCR (qPCR) is used to validate the key biological processes and signaling pathways.
After systematic biological analysis, two networks were constructed and analyzed: (1) TNBC network; (2) Baicalin-TNBC protein-protein interaction (PPI) network. Several TNBC-related, treatment-related targets, clusters, signaling pathways and biological processes were found. Cytology experiment shows that baicalin can inhibit the proliferation, migration and invasion of breast cancer MDA-MB-231 cells in vitro (P < 0.05). The results of qPCR showed that baicalin increase the expression of E-cadherin mRNA, and decrease the expression of vimentin, β-catenin, c-Myc and MMP-7 mRNA in LPS-induced breast cancer MDA-MB-231 cells (P < 0.05).
Baicalin may achieve anti-tumor effects through regulating the targets, biological processes and pathways found in this research.
通过系统生物学策略和细胞学实验研究黄芩苷对三阴性乳腺癌（TNBC）生物网络的调控机制。
利用系统生物学方法预测黄芩苷的潜在靶点，收集 TNBC 的基因，并分析 TNBC 和黄芩苷的网络。系统生物学分析完成后，采用实时定量 PCR（qPCR）验证关键的生物学过程和信号通路。
经过系统生物学分析，构建并分析了两个网络：（1）TNBC 网络；（2）黄芩苷-TNBC 蛋白-蛋白相互作用（PPI）网络。发现了几个与 TNBC 相关的、与治疗相关的靶点、簇、信号通路和生物学过程。细胞学实验表明，黄芩苷能抑制乳腺癌 MDA-MB-231 细胞的体外增殖、迁移和侵袭（P<0.05）。qPCR 结果显示，黄芩苷能增加 LPS 诱导的乳腺癌 MDA-MB-231 细胞中 E-钙黏蛋白 mRNA 的表达，降低波形蛋白、β-连环蛋白、c-Myc 和 MMP-7 mRNA 的表达（P<0.05）。
黄芩苷可能通过调节本研究中发现的靶标、生物学过程和途径发挥抗肿瘤作用。
