伊朗南部法尔斯省一个农村社区儿童中弓形虫感染率较低。

Low prevalence of Toxoplasma gondii infection among children in a rural community in Fars province, Southern Iran.

作者信息

Arefkhah Nasir, Goodarzi Roghayeh, Rezaei Zahra, Layegh Gigloo Akram, Sarkari Bahador

机构信息

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

出版信息

Infez Med. 2019 Sep 1;27(3):322-327.

PMID:31545777

Abstract

The current study aimed to determine the seroprevalence of Toxoplasma gondii infection and associated risk factors in children in a rural community in Fars province in southern Iran. Blood samples were collected from 671 children living in three rural areas, and sera and buffy coats were isolated from each sample. Anti-T. gondii antibodies were detected by ELISA, using a commercial kit. Also, buffy coats of seropositive children were examined by a PCR method, targeting a 529 bp gene of T. gondii. Of 671 children participating in the study, 319 (51.7%) were boys and 298 (48.3%) were girls. The mean age of the children was 9.7 (±10.7) years. Anti-Toxoplasma antibodies were detected in sera of 23 out of 671 children, corresponding to a seroprevalence rate of 3.8%. Gender and level of education had no significant influence on the risk of Toxoplasma infection (p>0.05). Toxoplasma DNA was not detected in buffy coats of any of the seropositive cases. Geographic location, as well as the low age of the participants, may contribute to the low rate of Toxoplasma infection in children of rural areas in southern Iran.

摘要

本研究旨在确定伊朗南部法尔斯省一个农村社区儿童弓形虫感染的血清流行率及相关危险因素。从三个农村地区的671名儿童采集血样，并从每个样本中分离出血清和血沉棕黄层。使用商用试剂盒通过酶联免疫吸附测定法（ELISA）检测抗弓形虫抗体。此外，采用针对弓形虫529 bp基因的聚合酶链反应（PCR）方法检测血清阳性儿童的血沉棕黄层。参与研究的671名儿童中，319名（51.7%）为男孩，298名（48.3%）为女孩。儿童的平均年龄为9.7（±10.7）岁。671名儿童中有23名血清中检测到抗弓形虫抗体，血清流行率为3.8%。性别和教育程度对弓形虫感染风险无显著影响（p>0.05）。在任何血清阳性病例的血沉棕黄层中均未检测到弓形虫DNA。地理位置以及参与者年龄较小可能导致伊朗南部农村地区儿童弓形虫感染率较低。