Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
Comp Immunol Microbiol Infect Dis. 2019 Oct;66:101342. doi: 10.1016/j.cimid.2019.101342. Epub 2019 Jul 30.
Given the lack of routine screening and the high prevalence of toxoplasmosis in pregnant women in Iran, the current study aimed to find out the rate and features of Toxoplasma gondii infection in the spontaneously aborted human fetuses in Kohgiluyeh and Boyer-Ahmad Province, Southwestern Iran.
This cross-sectional study was performed on 100 spontaneously aborted fetuses' tissues and their mother blood samples. The mothers' sera were evaluated for anti-Toxoplasma antibodies while their buffy coat and aborted fetuses tissues were evaluated for Toxoplasma DNA. PCR product at GRA6 locus was sequenced and phylogenetic analysis was done. Likewise, quantitative Real-Time PCR was performed to find out the parasite burdens in mothers buffy coat and fetuses tissues.
Using serological method, anti-Toxoplasma IgG and IgM antibodies were detected in 7 (7%) and 3 (3%) out of 100 sera from women with spontaneous abortion. Real-time PCR method detected T. gondii DNA in the buffy coat of one seronegative and 2 (out of 3) IgM seropositive cases. None of the samples from aborted fetuses were infected with T. gondii. BLAST and phylogenetic analysis showed that the sequenced isolates belonged to type I of T. gondii and two identified T. gondii isolates were taxonomically grouped into one clade.
Our findings revealed type I genotype of T. gondii in two mothers with spontaneous abortion, without fetus involvement. It is necessary to examine more aborted fetuses' samples from different geographical areas to determine the association between Toxoplasma genotype and abortion.
鉴于伊朗孕妇常规筛查的缺乏和弓形虫感染的高流行率，本研究旨在了解伊朗西南部戈尔甘和博耶-艾哈迈德省自然流产的人类胎儿中弓形虫感染的发生率和特征。
本横断面研究对 100 例自然流产胎儿组织及其母亲的血样进行了研究。评估母亲血清中的抗弓形虫抗体，同时评估其白细胞层和流产胎儿组织中的弓形虫 DNA。对 GRA6 基因座的 PCR 产物进行测序，并进行系统发育分析。同样，进行定量实时 PCR 以确定母亲白细胞层和胎儿组织中的寄生虫负荷。
使用血清学方法，在 100 例自然流产妇女的血清中，有 7 例（7%）和 3 例（3%）检测到抗弓形虫 IgG 和 IgM 抗体。实时 PCR 方法在 1 例血清阴性和 2 例（3 例中的 2 例）IgM 血清阳性病例的白细胞层中检测到 T. gondii DNA。流产胎儿的样本均未感染弓形虫。BLAST 和系统发育分析表明，测序分离株属于弓形虫 I 型，两种鉴定的弓形虫分离株在分类上归入一个分支。
我们的研究结果显示，两名自然流产的母亲携带 I 型基因型的弓形虫，但未感染胎儿。有必要从不同地理区域检查更多流产胎儿的样本，以确定弓形虫基因型与流产之间的关系。
